[Effects of enamel matrix proteins on the attachment,spreading and proliferation of porcine bone marrow stromal cells in vitro].

Purpose: The purpose of this study is to investigate the influence of enamel matrix proteins(EMPs) on the attachment, spreading and proliferation of cultured porcine bone marrow stromal cells (BMSCs).

Methods: BMSCs were obtained from porcine bone marrow aspiration and cultured in DMEM medium with 10% FBS.The third passage cells were exposed to various concentrations of EMPs (25,50,100 and 200microg /ml).Controls were BMSCs cultured in DMEM medium without EMPs. The cell attachment was analyzed by a colorimetric assay and cell spreading rates were performed at culture time of 1h, 3.5h and 6.5h by analysis of micrographs taken at predetermined sites of each wells. BMSCs proliferation rates were carried out over a 9-day period and assessed by an MTT assay. A parametric one-way analysis of variance (ANOVA) and multiple comparisons were used to test the treatments based on the Stument-Newman- Keuls test.

Results: It was shown that BMSCs well attached and spread on EMPs-coated substrata,however, there were no significant differences between the control group and experimental groups with various concentrations of EMPs. The proliferation of BMSCs was significantly stimulated by EMPs in a dose- and time- dependent manner,and EMPs at a concentration of 200microg/ml significantly enhanced BMSCs proliferation from day 3 over the experiment.

Conclusions: The results demonstrate that EMPs stimulate BMSCs proliferation, whereas this factor does not affect the attachment and spreading of these cells. These findings lend theoretical support to the combined application of EMPs and BMSCs in repairing periodontal defects.