AI Article Synopsis
- The compound 2-(4-dimethylaminophenyl)-5-fluoro-6-(morpholin-4-yl)-1H-benzimidazole (1) can act as a tertiary base, able to bind up to three protons, as shown by UV-Vis spectra at various pH levels.
- Through analysis, the three protonation constants of compound 1 were calculated, with values around 4.96, 5.72, and 7.95.
- The compound shows strong interaction with calf thymus DNA at pH 3.40, which indicates its potential use for quantitative DNA detection in very low concentrations (10^-8 to 1.2 x 10^-6 mol x
Article Abstract
UV-Vis spectra of the aqueous solutions of 2-(4-dimethylaminophenyl)-5-fluoro-6-(morpholin-4-yl)-1H-benzimidazole (1) at different pH values revealed that compound 1 is a tertiary base, which can combine three protons. Through the non-linear least square method, the logarithm of the three accumulative protonation constants of compound 1, namely, lgbeta1, lgbeta2 and lgbeta3, were determined to be 4.96 +/- 0.03, 5.72 +/- 0.07 and 7.95 +/- 0.10, respectively. UV-Vis and the steady-state fluorescence spectra indicated that a special interaction occurs between compound 1 and calf thymus DNA at the pH value of 3.40, of which thebinding constant, Kb, is (2.30 +/- 0.10) x 10(4) mol(-1) x L. Compound 1 in the concentration range of 10(-8) to 1.2 x 10(-6) mol x L(-1) has a potential application to the quantitative determination of DNA.
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