The Moloney murine leukemia virus-transformed suspension cell line WMP2 is derived from wild mice (Mus musculus) of the WMP/WMP strain. These mice carry nine pairs of metacentric Robertsonian translocation chromosomes. As the chromosomes of the wild-type mouse are all acrocentric, metaphase spreads of the WMP2 cells seam to be highly suited for physical gene mapping. Here we studied the WMP2 line using spectral karyotyping (SKY) combined with new established mouse specific multicolor banding (mcb) probes for the chromosomes X, 3, 4, 6 and 18. SKY revealed that the WMP2 cell line developed further four derivative chromosomes. After application of mcb five previously unrecognizable intrachromosomal rearrangements with 9 breakpoints were detected for the studied chromosomes.
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Molecular cytogenetic characterization of the mouse cell line WMP2 by spectral karyotyping and multicolor banding applying murine probes.
Int J Mol Med
February 2006
Institute of Human Genetics and Anthropology, D-07743 Jena, Germany.
The Moloney murine leukemia virus-transformed suspension cell line WMP2 is derived from wild mice (Mus musculus) of the WMP/WMP strain. These mice carry nine pairs of metacentric Robertsonian translocation chromosomes. As the chromosomes of the wild-type mouse are all acrocentric, metaphase spreads of the WMP2 cells seam to be highly suited for physical gene mapping.
View Article and Find Full Text PDFSpectral karyotyping of mouse cell line WMP2.
Cytogenet Cell Genet
February 2001
Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, MI 48202, USA.
We have evaluated the mouse cell line WMP2 using both GTG-banding analysis and spectral karyotyping to verify the reliability of using this established cell line derived from WMP/WMP mice. The WMP cell lines contain easily identifiable metacentric fusion chromosomes and are used extensively for gene mapping. Because of karyotypical changes in the WMP1 cell line, WMP2 was examined.
View Article and Find Full Text PDFEstablishment of permanent wild-mouse cell lines with readily identifiable marker chromosomes.
Cytogenet Cell Genet
August 1995
Institut für Molekularbiologie und Tumorforschung, IMT, Universität Marburg, Germany.
Physical gene mapping by in situ hybridization is a difficult task in an all-acrocentric mouse karyotype, because all of the chromosomes are morphologically very similar. These difficulties can be overcome by using the many different metacentric Robertsonian translocation (Rb) chromosomes derived from wild mice. Here we describe the establishment of two Moloney murine leukemia virus-transformed suspension cell lines, WMP-1 and WMP-2, derived from wild mice of the strain WMP/WMP.
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