An oligopeptidase from Bacillus amyloliquefaciens 23-7A was characterized along with its biochemical activities and structural gene. The protein's amino acid sequence and enzymatic activities were similar to those of other bacterial PepFs, which belong to metallopeptidase family M3. While most bacterial PepFs are cytoplasmic endopeptidases, the identified PepFBa oligopeptidase is a secreted protein and may facilitate the process of sporulation.
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http://dx.doi.org/10.1128/AEM.72.1.968-971.2006 | DOI Listing |
BMC Genomics
December 2024
Institute of Biology (IB), State University of Campinas (UNICAMP), Campinas, SP, Brazil.
Background: Elucidating the intricacies of the sugarcane genome is essential for breeding superior cultivars. This economically important crop originates from hybridizations of highly polyploid Saccharum species. However, the large size (10 Gb), high degree of polyploidy, and aneuploidy of the sugarcane genome pose significant challenges to complete genome sequencing, assembly, and annotation.
View Article and Find Full Text PDFAnal Chem
December 2024
Institute of Microbiology of the Czech Academy of Sciences, BioCeV, Videnska 1083, Prague 4 14220, Czechia.
In proteomics, postproline cleaving enzymes (PPCEs), such as prolyl endopeptidase (PEP) and neprosin, complement proteolytic tools because proline is a stop site for many proteases. But while aiming at using PEP in online proteolysis, we found that this enzyme also displayed specificity to reduced cysteine. By LC-MS/MS, we systematically analyzed PEP sources and conditions that could affect this cleavage preference.
View Article and Find Full Text PDFInt J Biol Macromol
December 2024
Beamline Development and Application Section, Bhabha Atomic Research Centre, Mumbai 400085, Maharashtra, India. Electronic address:
Int J Biol Macromol
November 2024
Dr. Ikram ul Haq Institute of Industrial Biotechnology, Government College University, Lahore, Pakistan.
Sci Rep
August 2024
National Centre for Biological Sciences (TIFR), GKVK Campus, Bangalore, Karnataka, 560065, India.
Celiac disease (CD) is a common autoimmune disorder in which the patients are unable to digest gluten, which is present in foods made up of wheat, barley and rye. Whilst diagnosis happens late in 80% of the cases, avoidance of such foods appears to be the common solution. Alternative management strategies are required for the patients and their families since CD is also genetically carried over.
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