The acid waters (pH 2.7 to 3.4) originating from the Carnoulès mine tailings contain high concentrations of dissolved arsenic (80 to 350 mg.liter(-1)), iron (750 to 2,700 mg.liter(-1)), and sulfate (2,000 to 7,500 mg.liter(-1)). During the first 30 m of downflow in Reigous creek issuing from the mine tailings, 20 to 60% of the dissolved arsenic is removed by coprecipitation with Fe(III). The microbial communities along the creek have been characterized using terminal-restriction fragment length polymorphism (T-RFLP) and 16S rRNA gene library analyses. The results indicate a low bacterial diversity in comparison with unpolluted water. Eighty percent of the sequences obtained are related to sequences from uncultured, newly described organisms or recently associated with acid mine drainage. As expected owing to the water chemistry, the sequences recovered are mainly related to bacteria involved in the geochemical Fe and S cycles. Among them, sequences related to uncultured TrefC4 affiliated with Gallionella ferruginea, a neutrophilic Fe-oxidizing bacterium, are dominant. The description of the bacterial community structure and its dynamics lead to a better understanding of the natural remediation processes occurring at this site.
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http://dx.doi.org/10.1128/AEM.72.1.551-556.2006 | DOI Listing |
Biotechnol Notes
December 2024
Centre for Molecular Biology, Central University of Jammu, Rahya Suchani (Bagla), Jammu & Kashmir, India.
The amidases (EC 3.5.1.
View Article and Find Full Text PDFPlant Cell Environ
January 2025
Civil, Environmental, and Mining Engineering, University of Western Australia, Perth, Western Australia, Australia.
Understanding and predicting plant water dynamics during and after water stress is increasingly important but challenging because the high-dimensional nature of the soil-plant-atmosphere system makes it difficult to identify mechanisms and constrain behaviour. Datasets that capture hydrological, physiological and meteorological variation during changing water availability are relatively rare but offer a potentially valuable resource to constrain plant water dynamics. This study reports on a drydown and re-wetting experiment of potted Populus trichocarpa, which intensively characterised plant water fluxes, water status and water sources.
View Article and Find Full Text PDFEnviron Sci Technol
January 2025
Marine Synthetic Ecology Research Center, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Guangdong Provincial Observation and Research Station for Marine Ranching in Lingdingyang Bay, China-ASEAN Belt and Road Joint Laboratory on Mariculture Technology, State Key Laboratory for Biocontrol, Sun Yat-sen University, Zhuhai 519082, China.
Plants can recruit microorganisms to enhance soil arsenic (As) removal and nitrogen (N) turnover, but how microbial As methylation in the rhizosphere is affected by N biotransformation is not well understood. Here, we used acetylene reduction assay, gene amplicon, and metagenome sequencing to evaluate the influence of N biotransformation on As methylation in the rhizosphere of , a potential As hyperaccumulator. was grown in mining soils (MS) and artificial As-contaminated soils (AS) over two generations in a controlled pot experiment.
View Article and Find Full Text PDFAdv Sci (Weinh)
January 2025
School of Low-Carbon Energy and Power Engineering, China University of Mining and Technology, NO. 1 DAXUE ROAD, Xuzhou, Jiangsu, 221116, China.
With the increasing demand for thermal management, phase change materials (PCMs) have garnered widespread attention due to their unique advantages in energy storage and temperature regulation. However, traditional PCMs present challenges in modification, with commonly used physical methods facing stability and compatibility issues. This study introduces a simple and effective chemical method by synthesizing seven ester-based PCMs through chemical reactions involving lauric acid (LA) and seven different alcohols.
View Article and Find Full Text PDFAnal Chim Acta
February 2025
School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan, 450001, China. Electronic address:
Background: DNA methylation catalyzed by various DNA methyltransferases (DNA MTases) is one of the important epigenetic regulations in both eukaryotes and prokaryotes. Therefore, the detection of DNA MTase activity is a vital target and direction in the study of methylation-related diseases.
Results: In this study, an ultrasensitive and robust strategy was developed for DNA MTase activity sensing based on bifunctional probe propelling multipath strand displacement amplification and CRISPR/Cas12a techniques.
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