Safe Mycobacterium tuberculosis DNA extraction method that does not compromise integrity.

J Clin Microbiol

DST/NRF Centre of Excellence in Biomedical Tuberculosis Research, MRC Centre for Molecular and Cellular Biology, Department of Medical Biochemistry, Faculty of Health Sciences, Stellenbosch University, Tygerberg, South Africa.

Published: January 2006

Concern has been raised about the efficacy of the heat killing of mycobacteria during the isolation of DNA. We demonstrate a method that allows for the efficient killing of Mycobacterium tuberculosis without compromising DNA integrity for subsequent molecular investigation. This method reduces the risk of infection to the research scientist.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1351970PMC
http://dx.doi.org/10.1128/JCM.44.1.254-256.2006DOI Listing

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