AI Article Synopsis
- A multilaboratory study compared the Assurance GDS methods for detecting E. coli 0157:H7 and Shigatoxin Genes against standard culture methods in orange juice, raw ground beef, and fresh lettuce.
- Fifteen laboratories participated, analyzing the effectiveness of these methods through Chi square analysis at varying contamination levels.
- Results showed that both Assurance GDS methods performed equally well or better than traditional reference methods across all tested food types.
Article Abstract
A multilaboratory collaborative study was conducted to compare the Assurance GDS for E. coli 0157:H7 method and the reference culture methods for the detection of E. coli 0157:H7 in orange juice, raw ground beef, and fresh lettuce. A separate companion assay, the Assurance GDS for Shigatoxin Genes method was also evaluated with the same test portions. Fifteen laboratories participated in the study. A Chi square analysis of each of the 3 food types at the high, low, and uninoculated control levels was performed. For all foods, the Assurance GDS for E. coli O157:H7 method and the Assurance GDS for Shigatoxin Genes method were equivalent to or better than the reference methods.
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