AI Article Synopsis
- Genetic engineering often requires multiple genes to be introduced simultaneously, which can be difficult using traditional methods.
- The 2A oligopeptide offers an efficient solution by allowing multiple proteins to be expressed from a single gene linked by 2A sequences, enabling co-expression during transcription.
- This technique has proven effective across various eukaryotic systems and has been successfully applied in diverse biotechnological fields, including plant metabolism and medical applications like antibody production.
Article Abstract
Many applications of genetic engineering require transformation with multiple (trans)genes, although to achieve these using conventional techniques can be challenging. The 2A oligopeptide is emerging as a highly effective new tool for the facile co-expression of multiple proteins in a single transformation step, whereby a gene encoding multiple proteins, linked by 2A sequences, is transcribed from a single promoter. The polyprotein self-processes co-translationally such that each constituent protein is generated as a discrete translation product. 2A functions in all the eukaryotic systems tested to date and has already been applied, with great success, to a broad range of biotechnological applications: from plant metabolome engineering to the expression of T-cell receptor complexes, monoclonal antibodies or heterodimeric cytokines in animals.
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| http://dx.doi.org/10.1016/j.tibtech.2005.12.006 | DOI Listing |
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