Ochratoxin A (OA) is a nephrotoxic and carcinogenic mycotoxin that has been found in cereal and food commodities. Currently, Aspergillus carbonarius, A. niger and A. ochraceus have been recognized as the species responsible for OA in coffee beans. With the aim of developing multiplex-PCR for detection of these species in coffee bean samples, we first developed specific primers for A. niger detection. The primer designed (OPX7(372)) provided an amplicon of 372 pb in all A. niger stricto sensu isolates. The PCR assay developed for A. niger identification in pure culture was also successfully used for detecting this species in coffee beans. No cross-reaction was observed using DNA from coffee beans inoculated with closely related black aspergilli species. A multiplex-PCR method for detection of A. carbonarius, A. niger and A. ochraceus species in coffee beans was developed. From a single assay this method detected the amplicons of 809, 372, and 260 pb that represent the three most ochratoxigenic species found in coffee bean samples, i.e., A. carbonarius, A. niger and A. ochraceus, respectively.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.resmic.2005.09.008 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Current status of toxicological research on stingless bees (Apidae, Meliponini): Important pollinators neglected by pesticides' regulations.
Sci Total Environ
December 2024
Sociedad Latinoamericana de Investigación en Abejas (SoLatInA), Montevideo, Uruguay; Laboratorio de Microbiología y Salud de las Abejas, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Avda. Italia 3318, Montevideo, Uruguay; Centro de Investigación en Ciencias Ambientales (CICA), Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Avda. Italia 3318, Montevideo, Uruguay. Electronic address:
Stingless bees (tribe Meliponini), comprising over 600 known species within the largest group of eusocial bees, play a critical role in ecosystem functioning through their pollination services. They contribute to the reproduction of numerous plant species, including many economically important crops such as cacao, coffee, and various fruits. Beyond their ecological significance, stingless bees hold cultural and economic importance for many native and rural communities, where they are managed for their honey, pollen, and propolis for nutritional and health purposes.
View Article and Find Full Text PDFKnown from trees and the tropics: new insights into the species complex.
Stud Mycol
December 2024
Westerdijk Fungal Biodiversity Institute, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.
The species complex (FLSC) currently comprises 11 phylogenetic species, including accepted names such as , , and , which have mostly been reported in association with citrus and coffee. Many varieties were documented by Wollenweber & Reinking (1935), which is indicative of a wider diversity of species within this group. The lack of type material in some cases, especially for the older names, means that definition by molecular phylogeny is very difficult.
View Article and Find Full Text PDFThe Identification and Characterization of WOX Family Genes in Reveals Their Potential Roles in Somatic Embryogenesis and the Cold-Stress Response.
Int J Mol Sci
December 2024
School of Agriculture, Yunnan University, Kunming 650500, China.
() genes play significant roles in plant development and stress responses. Difficulties in somatic embryogenesis are a significant constraint on the uniform seedling production and genetic modification of , hindering efforts to improve coffee production in Yunnan, China. This study comprehensively analyzed genes in three species.
View Article and Find Full Text PDFComparative Analysis of Phytochemical and Functional Profiles of Arabica Coffee Leaves and Green Beans Across Different Cultivars.
Foods
November 2024
College of Veterinary Medicine, Jeju National University, Jeju 63243, Republic of Korea.
This study analyzed the phytochemical composition and functional properties of leaves and green beans from seven Arabica coffee cultivars. The total phenolic and flavonoid contents were measured using spectrophotometric methods, while caffeine, chlorogenic acid (CGA), and mangiferin levels were quantified via High-Performance Liquid Chromatography (HPLC). Volatile compounds were identified using Gas Chromatography-Mass Spectrometry (GC-MS).
View Article and Find Full Text PDFMaxEnt and Marxan modeling to predict the potential habitat and priority planting areas of in Yunnan, China under climate change scenario.
Front Plant Sci
November 2024
Foreign Environmental Cooperation Center, Ministry of Ecology and Environment, Beijing, China.
Introduction: (Arabica coffee) is an important cash crop in Yunnan, China. Ongoing climate change has made coffee production more difficult to sustain, posing challenges for the region's coffee industry. Predictions of the distribution of potentially suitable habitats for Arabica coffee in Yunnan could provide a theoretical basis for the cultivation and rational management of this species.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!