During mammalian development, expression of the Nephroblastoma overexpressed gene (NOV/CCN3) is tightly regulated in skeletal muscles. Ex vivo, ectopic expression of NOV blocks myogenic differentiation. NOV also supports endothelial cell adhesion and angiogenesis through interactions with integrins. Integrins play fundamental roles during myogenesis. In this study, we show that NOV mediates adhesion and spreading of myoblasts. Myoblasts adhesion to NOV does not require proteoglycans and is dependent on integrin beta1, whereas spreading involves another RGD-sensitive integrin. The C-Terminal part of NOV as well as full-length is able to support adhesion of myoblasts; in addition, both increase focal-adhesion kinase (FAK) phosphorylation. Furthermore, NOV is an adhesive substrate that, combined with FGF2 or IGF-1, promotes cell specific proliferation and survival, respectively, in a better way than fibronectin. Taken together, these results identify NOV as an adhesion substrate for myoblasts which, in concert with growth factors, could play a role in the physiology of muscle cells.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1080/15419060500383069 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Clinical effect of acupuncture on knee osteoarthritis and its effect on p38 MAPK signaling pathway.
Turk J Phys Med Rehabil
September 2024
Department of Needle Knife, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, China.
Objectives: This study aims to investigate the curative efficacy of acupuncture on knee osteoarthritis (KOA) and its improvement on related scores and blood indexes.
Patients And Methods: Between January 2019 and January 2020, a total of 108 patients (48 males, 60 females; mean age: 61.0±6.
Specific Activation of the Expression of Growth Factor Genes in Expi293F Human Cells Using CRISPR/Cas9-SAM Technology Increases Their Proliferation.
Acta Naturae
January 2024
Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow,119435 Russian Federation.
Human cell lines play an important role in biotechnology and pharmacology. For them to grow, they need complex nutrient media containing signaling proteins - growth factors. We have tested a new approach that reduces the need of cultured human cell lines for exogenous growth factors.
View Article and Find Full Text PDFRecent advances in the use of essential oils and their nanoformulations for wound treatment.
Burns
February 2025
National Agency of Medicinal and Aromatic Plants, 34025 Taounate, Morocco.
Article Synopsis
- Bioactive compounds, particularly essential oils (EOs) and their terpenoids, have been shown to effectively accelerate wound healing (WH) by providing a safe alternative to traditional treatments.
- Studies have identified 38 species of EOs from various families, with the Lamiaceae family being the most represented, highlighting their roles as anti-inflammatory, antioxidant, and antimicrobial agents, and facilitating cellular repair and proliferation through various biological mechanisms.
- Recent advancements in nanotechnology have enhanced the development of drug delivery systems (DDS) that incorporate EOs, but challenges remain in translating these innovations into practical clinical applications for wound management.
The effects of fraxetin and monnieriside A on Cultured L929 fibroblasts.
Nat Prod Res
June 2024
CETENS, Federal University of Recôncavo da Bahia, Feira de Santana, Brazil.
Skin lesions are considered a public health problem, compromising patients' quality of life. This work aimed to evaluate the effects of fraxetin and monnieriside A on Cultured L929 Fibroblasts through the scratch assay. Supernatants and cells from the fibroblast culture treated with the compounds were used to evaluate essential markers of the tissue repair process (IGF-1, VEGF, IL-8, IL-10, FGF-2, COL1A2, COL4A, PDGF) using ELISA and qRT-PCR.
View Article and Find Full Text PDFIn this article, we focused on the impact of precisely chemically modified FLI maturation medium enriched with fibroblast growth factor 2 (FGF2), leukemia inhibitory factor (LIF), insulin-like growth factor 1 (IGF1), and polyvinyl alcohol (PVA) and its potential to improve the efficiency of in vitro production of porcine embryos. We hypothesized that enhancing the composition of the maturation medium could result in an elevated production of embryos in vitro and can affect EGA. FLI medium resulted in a significantly higher rate of oocyte blastocyst maturation and formation compared to the control DMEM medium.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!