AI Article Synopsis
- - The study aimed to compare the protein differences between two strains of Bartonella henselae: Houston and Marseille, using two-dimensional gel electrophoresis.
- - Protein samples were prepared and analyzed, showing similar concentrations (2.117 microg/microL for Houston and 2.200 microg/microL for Marseille), with 95% of protein spots being identical between the two strains.
- - The findings suggest that while the two strains have minor protein differences, the 2-DE method is effective for proteomic analysis, indicating that they represent different genotypes of Bartonella henselae.
Article Abstract
Objective: To compare the protein difference between B. henselae Houston and B. henselae Marseille by two-dimensional gel electrophoresis.
Method: Protein samples were prepared by vorterx, ultrasonic treatment, and centrifugation. Protein concentrations were determined by Bradford method. Protein difference was compared by the first IEF and the second SDS-polyacrylamide gel electrophoresis.
Results: Protein concentrations in samples of Bartonella henselae Houston and Bartonella henselae Marseille were 2.117 microg/microL and 2.200 microg/microL respectively. Sample protein of 40 microg for IPG strips loading was perfect. The results of 2-DE in pH 4 to 7 IPG strips showed that the total protein spots of Bartonella henselae Houston and Bartonella henselae Marseille were 375 and 379 respectively, 95% of the spots were the same between the two strains of Bartonella henselae.
Conclusion: The procedure of 2-DE may prove successful for the proteomic analysis of Bartonella henselae. Bartonella henselae Houston and Bartonella henselae Marseille are different genotypes.
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