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Evaluation of nationwide analysis surveillance for methicillin-resistant within Genomic Medicine Sweden.
Microb Genom
January 2025
Department of Laboratory Medicine, Clinical Microbiology, Faculty of Medicine and Health, rebro University, rebro, Sweden.
National epidemiological investigations of microbial infections greatly benefit from the increased information gained by whole-genome sequencing (WGS) in combination with standardized approaches for data sharing and analysis. To evaluate the quality and accuracy of WGS data generated by different laboratories but analysed by joint pipelines to reach a national surveillance approach. A national methicillin-resistant (MRSA) collection of 20 strains was distributed to nine participating laboratories that performed in-house procedures for WGS.
View Article and Find Full Text PDFFrom isolation to detection, advancing insights into endothelial matrix-bound vesicles.
Extracell Vesicle
December 2024
Department of Chemical Engineering, University of Puerto Rico-Mayaguez, Route 108, Mayaguez, Puerto Rico, USA.
Matrix-bound vesicles (MBVs), an integral part of the extracellular matrix (ECM), are emerging as pivotal factors in ECM-driven molecular signaling. This study is the first to report the isolation of MBVs from porcine arterial endothelial cell basement membranes (A-MBVs) and thyroid cartilage (C-MBVs), the latter serving as a negative control due to its minimal vascular characteristics. Using Transmission Electron Microscopy (TEM), Nano-Tracking Analysis (NTA), Electrochemical Impedance Spectroscopy (EIS), and Atomic Force Microscopy (AFM), we orthogonally characterized the isolated MBVs.
View Article and Find Full Text PDFHighly Green Fluorescent Carbon Dots from Gallic Acid: A Turn-On Sensor toward Pb Ions.
ACS Omega
January 2025
Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia.
Carbon dots (CDs) are emerging novel fluorescent sensing nanomaterials owing to their tunable optical properties, biocompatibility, and eco-friendliness. Herein, we report a facile one-pot hydrothermal route for the synthesis of highly green fluorescent CDs using gallic acid (GA) as a single carbon source in ,-dimethylformamide (DMF) solvent, which serves as a nitrogen source and reaction medium. The optical properties of the synthesized GA-DMF CDs were systematically characterized by using UV-vis and photoluminescence spectroscopy, revealing strong green fluorescence.
View Article and Find Full Text PDFResidence time of particles in indoor surface networks.
J Hazard Mater
January 2025
Department of Mechanical Engineering, The University of Hong Kong, Hong Kong, PR China; Faculty of Architecture, The University of Hong Kong, Hong Kong, PR China. Electronic address:
Infectious microbes can spread rapidly from fomites (contaminated surfaces) via hand touch, with prolonged residence time on surfaces increasing transmission risk by extending exposure periods and/or involving more susceptible individuals. Existing studies have focused on decreasing microbial contamination, but not on the need for rapid removal from surface systems. This study introduces residence time as the time that a microbe spends within the surface system.
View Article and Find Full Text PDFRisk Factors and Circulating Subtypes of spp. and in Hospitalized Children in Mozambique.
Microorganisms
January 2025
Unidade de Parasitologia Médica, Grupo de Parasitas Oportunistas/VIH e Outros Parasitas, Global Health and Tropical Medicine, GHTM, Associate Laboratory in Translation and Innovation Towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical, IHMT, Universidade NOVA de Lisboa, 1349-008 Lisboa, Portugal.
spp. and are important diarrheal agents in children in developing countries. Little is known about their molecular epidemiology; as such, the objective of this study was to determine the risk factors and genetic diversity of both parasites in diarrheal samples in Mozambique.
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