Despite the availability of high-throughput transcript profiling technology, little is known about tissue-specific gene expression patterns in the wood-forming tissues of Eucalyptus plantation tree species. We used cDNA-amplified fragment length polymorphism (AFLP) analysis in combination with infrared fragment detection and semi-automated band quantification to profile gene expression in a 6-year-old, fast- growing Eucalyptus tree. The expression profiles of 6385 transcript-derived fragments (TDFs) were analyzed across four major woody tissues (mature xylem, immature xylem, phloem and cork) collected from two stem positions, to provide a global view of transcript abundance and variability in the Eucalyptus stem. About 21% of the TDFs were differentially expressed and could be grouped into clusters representing co- expressed genes. A total of 71 TDFs representing different gene clusters were isolated and characterized. These included genes implicated in cell fate, signal transduction and cell wall biosynthesis, processes closely associated with xylogenesis. Analysis of the expression levels of selected TDFs by quantitative RT-PCR corroborated the TDF quantification and confirmed that cDNA-AFLP analysis is a highly efficient and accurate tool for transcript profiling and gene discovery in wood-forming tissues of tree species.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1093/treephys/26.3.365 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A systems genetic analysis identifies putative mechanisms and candidate genes regulating vessel traits in poplar wood.
Front Plant Sci
May 2024
USDA Forest Service, Pacific Southwest Research Station, Davis, CA, United States.
Wood is the water conducting tissue of tree stems. Like most angiosperm trees, poplar wood contains water-conducting vessel elements whose functional properties affect water transport and growth rates, as well as susceptibility to embolism and hydraulic failure during water stress and drought. Here we used a unique hybrid poplar pedigree carrying genomically characterized chromosomal insertions and deletions to undertake a systems genomics analysis of vessel traits.
View Article and Find Full Text PDFFlexure wood formation via growth reprogramming in hybrid aspen involves jasmonates and polyamines and transcriptional changes resembling tension wood development.
New Phytol
December 2023
Umeå Plant Science Centre (UPSC), Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, 90183, Umeå, Sweden.
Stem bending in trees induces flexure wood but its properties and development are poorly understood. Here, we investigated the effects of low-intensity multidirectional stem flexing on growth and wood properties of hybrid aspen, and on its transcriptomic and hormonal responses. Glasshouse-grown trees were either kept stationary or subjected to several daily shakes for 5 wk, after which the transcriptomes and hormones were analyzed in the cambial region and developing wood tissues, and the wood properties were analyzed by physical, chemical and microscopy techniques.
View Article and Find Full Text PDFField evaluation of transgenic hybrid poplars with desirable wood properties and enhanced growth for biofuel production by bicistronic expression of PdGA20ox1 and PtrMYB3 in wood-forming tissue.
Biotechnol Biofuels
September 2021
Department of Plant & Environmental New Resources, Kyung Hee University, 446-701, Yongin, Republic of Korea.
Article Synopsis
- Researchers engineered a woody bioenergy crop by using a specific gene expression strategy to enhance growth and biomass properties through the combination of two genes, PtrMYB3 and PdGA20ox1.
- The transgenic plants demonstrated substantial increases in weight and growth metrics compared to wild-type plants, with Arabidopsis showing threefold increases in stem weight and poplars showing up to 2.6-fold increases in greenhouse conditions.
- Overall, the transgenic poplars exhibited enhanced wood formation and quality, making them promising candidates for biofuel production despite no significant changes in secondary wall thickness.
Expression and localization of SWEETs in Populus and the effect of SWEET7 overexpression in secondary growth.
Tree Physiol
May 2021
State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China.
In trees, wood formation needs carbon import from the photosynthetic source tissues. Sugar transporters play important roles in carbohydrate transport into wood-forming cells. Sugars will eventually be exported transporters (SWEETs) play essential roles in many physiological processes.
View Article and Find Full Text PDFWood properties and transcriptional responses of poplar hybrids in mixed cropping with the nitrogen-fixing species Robinia pseudoacacia.
Tree Physiol
May 2021
Abteilung für Forstbotanik und Baumphysiologie, Büsgen-Institut, Georg-August Universität, Büsgenweg 2, Göttingen 37077, Germany.
Cultivation of fast-growing tree species is often confined to marginal land. Mixed cropping with Robinia pseudoacacia, a legume tree species that forms a symbiosis with N2-fixing bacteria, has been proposed to be a measure to improve soil fertility and to achieve beneficial effects on the cocultivated tree species. The goal of our study was to examine the influence of a Robinia mixture on wood chemistry, anatomy and gene expression in poplar.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!