A yeast two-hybrid screening system was developed to screen for small molecules that inhibit the interaction of the Ras and the Raf proteins. Hyperpermeable yeast strains useful for high-throughput screening (HTS) for the two-hybrid system were created. Differential inhibition of the Ras-Raf vs the hsRPB4-hsRPB7 interaction allowed the identification of selective inhibitors.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7177006 | PMC |
| http://dx.doi.org/10.1007/978-1-59259-948-6_18 | DOI Listing |
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