Hematoxylin and eosin (H&E) staining of cutaneous warts is presented to illustrate the practical methods utilized for histochemical analysis of cutaneous human papillomavirus-associated lesions. Every step of the staining procedure, from sampling of the specimens to microscopic examination of the stained sections, is detailed with reference to the recent achievements in this field.
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, Encoding a Leucine-Rich Repeat Containing Receptor-like Protein, Is a Major Aphid () Resistance Gene in Sorghum.
Int J Mol Sci
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USDA-ARS Plant Science Research Laboratory, 1301N, Western Rd, Stillwater, OK 74075, USA.
Greenbug, , is one of the important cereal aphid pests of sorghum in the United States and other parts of the world. variety PI 607900 carries the resistance () gene that underlies plant resistance to greenbug biotype I (GBI). Now, the has been determined as the major gene conferring greenbug resistance based on the strong association of its presence with the resistance phenotype in sorghum.
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Microbiol Spectr
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Department of Pathology, Brigham and Women's Hospital, Boston, Massachusetts, USA.
is a vector of several human pathogens in the United States, including the cause of Lyme disease, and Powassan virus (POWV), an emerging cause of severe encephalitis. Skin biopsies from tick bite sites are frequently collected and tested for the presence of spirochetes ( spp.), which remain elusive.
View Article and Find Full Text PDFHistochemical indications for a chemically complex signal produced by the cervical gill slit gland of the pygmy sperm whale (Kogia breviceps).
Anat Rec (Hoboken)
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Department of Biology and Marine Biology, University of North Carolina Wilmington, Wilmington, North Carolina, USA.
The pygmy sperm whale (Kogia breviceps) possesses an exocrine gland associated with its false gill slit pigmentation pattern. The cervical gill slit gland is a compound tubuloalveolar gland that produces a holocrine secretion and displays maturational changes in size and secretory histology. While the morphology of the cervical gill slit gland has been described in detail, to date, the chemical composition of its secretion remains uncharacterized.
View Article and Find Full Text PDFAlkaPhos: a novel fluorescent probe as a potential point-of-care diagnostic tool to estimate recurrence risk of meningiomas.
Neurosurg Rev
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Department of Neurosurgery, Saarland University Medical Center, Homburg, Germany.
Deletion of the short arm of chromosome 1 (1p) increases recurrence rates in meningiomas by up to 33%, regardless of tumor grade, correlating with absence of intracellular alkaline phosphatase enzyme activity. Current screening methods for 1p deletion like fluorescence in situ hybridization (FISH) and loss of heterozygosity (LOH) analysis are resource-intensive. This study evaluated AlkaPhos, a novel fluorescent probe, for detecting alkaline phosphatase in meningioma cells and compared findings with FISH, LOH, and histochemical analysis.
View Article and Find Full Text PDFAdipose-Derived Stromal Cell Conditioned Medium on Bone Remodeling: Insights from a 3D Osteoblast-Osteoclast Co-Culture Model.
Calcif Tissue Int
January 2025
Department of Neuroscience and Rehabilitation, University of Ferrara, 44121, Ferrara, Italy.
This study describes the potential of the conditioned medium (CM) from adipose-derived mesenchymal stromal cells (ASCs) to affect the response of bone cells and support bone remodeling. This was in particular assessed by an in vitro model represented by a 3D human osteoblast-osteoclast co-culture. It has been reported that the effects of ASCs are predominantly attributable to the paracrine effects of their secreted factors, that are present as soluble factors or loaded into extracellular vesicles.
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