Tissues soaked at low temperature in a trypsin-citrate solution, washed to remove enzymes, and disrupted by rapid mechanical vibrations gave yields of viable cells 1.6 to 5 times those from conventional procedures.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC376550 | PMC |
http://dx.doi.org/10.1128/am.24.3.506-507.1972 | DOI Listing |
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