Improved cell dispersal method by low-temperature incubation of tissue in a trypsin-citrate solution.

Appl Microbiol

Rijks Instituut voor de Volksgezondheid, Postbus 1, Bilthoven, The Netherlands.

Published: September 1972

Tissues soaked at low temperature in a trypsin-citrate solution, washed to remove enzymes, and disrupted by rapid mechanical vibrations gave yields of viable cells 1.6 to 5 times those from conventional procedures.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC376550PMC
http://dx.doi.org/10.1128/am.24.3.506-507.1972DOI Listing

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