Potassium dichromate and formalin reduced the viability of Cryptosporidium parvum oocysts as assessed by inclusion or exclusion of 4',6-diamidino-2-phenylindole (DAPI) and propidium iodide (PI) and excystation. Some formalin-treated oocysts containing dead sporozoites excluded PI; that this fluorogenic assay relies not solely upon exclusion of PI but also upon highlighting of sporozoite nuclei by DAPI is reiterated.
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http://dx.doi.org/10.1128/aem.59.12.4361-4362.1993 | DOI Listing |
Water Environ Res
January 2025
Laboratorio Nacional para la Investigación en Inocuidad Alimentaria (LANIIA), Centro de Investigación en Alimentación y Desarrollo (CIAD), Culiacán, Sinaloa, Mexico.
The reliance on agriculture in many nations has increased the use of treated wastewater for irrigation. However, reclaimed water still poses health risks from resistant pathogens like Cryptosporidium spp. Ozone, a strong disinfectant, has been used in water treatment.
View Article and Find Full Text PDFParasitol Res
November 2024
Osaka International Research Center for Infectious Diseases, Osaka Metropolitan University, Osaka, 545-8585, Japan.
Cryptosporidium is a waterborne pathogen that causes diarrhea in vertebrates and humans (mainly C. hominis and C. parvum).
View Article and Find Full Text PDFBiomolecules
September 2024
Biosputnik LLC., New York, NY 10002, USA.
Front Microbiol
October 2024
Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.
This study evaluated the effectiveness of anti-cryptosporidial agents nitazoxanide, halofuginone, the pyrazolopyridine analog KDU731, and paromomycin (PMC) in combating the significant zoonotic pathogen . The study utilized HCT-8 host cells to culture and fluorescent microscopy/quantitative PCR (qPCR) for detecting parasitic growth. The efficacy of the compounds was assessed by calculating their inhibitory concentrations (IC) against the total growth of at 48 h post-infection.
View Article and Find Full Text PDFWater Res
July 2024
State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin University, Changchun, China. Electronic address:
While waters might be contaminated by oocysts from >40 Cryptosporidium species, only viable oocysts of C. parvum and C. hominis truly pose the main health risk to the immunocompetent population.
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