Effects of Preservatives on Viability of Cryptosporidium parvum Oocysts.

Appl Environ Microbiol

Scottish Parasite Diagnostic Laboratory, Stobhill General Hospital, Springburn, Glasgow G21 3UW, and Division of Environmental Health, University of Strathclyde, Glasgow G1 1XQ, Scotland.

Published: December 1993

Potassium dichromate and formalin reduced the viability of Cryptosporidium parvum oocysts as assessed by inclusion or exclusion of 4',6-diamidino-2-phenylindole (DAPI) and propidium iodide (PI) and excystation. Some formalin-treated oocysts containing dead sporozoites excluded PI; that this fluorogenic assay relies not solely upon exclusion of PI but also upon highlighting of sporozoite nuclei by DAPI is reiterated.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC195912PMC
http://dx.doi.org/10.1128/aem.59.12.4361-4362.1993DOI Listing

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