A heterologous gene probe encoding the alpha and beta subunits of the Pseudomonas cepacia protocatechuate 3,4-dioxygenase (PCD) was used to detect its homolog in the genome of Bradyrhizobium japonicum USDA110. Three cosmid clones carrying a 2.2-kb BamHI insert showed high levels of PCD activity. SacI digestion of one of the genomic clones, pBjG17, produced a 2.5-kb insert DNA that complemented a PCD mutant of P. cepacia.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC182344 | PMC |
| http://dx.doi.org/10.1128/aem.59.8.2717-2719.1993 | DOI Listing |
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