Direct determination of nitrification in marine waters by using the short-lived radioisotope of nitrogen, N.

Appl Environ Microbiol

Center for Environmental and Estuarine Studies, Chesapeake Biological Laboratory, Solomons, Maryland 20688-0038; Marine Sciences Research Center, State University of New York, Stony Brook, New York 11794 ; and Division of Chemistry, Brookhaven National Laboratory, Upton, New York 11973.

Published: April 1990

Biological oxidation of radiolabeled NH(4) (half-life = 10 min) was observed within minutes in assays of an estuarine ammonium oxidizer and in natural populations of nitrifiers in coastal waters. Our estimates of turnover of the ammonium pool and rates of nitrification based on experiments using N are consistent with previous values in the literature based on longer-term N tracer experiments or on indirect methods and thus provide corroboration for the estimates by other researchers.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC184368PMC
http://dx.doi.org/10.1128/aem.56.4.1182-1184.1990DOI Listing

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