A method is described for the purification of Bacillus thuringiensis protein crystals by Ludox gradient centrifugation. This method is simple, inexpensive, fast, and efficient compared with other techniques. It has been successfully used to purify and characterize the protein crystals from several B. thuringiensis strains.
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http://dx.doi.org/10.1128/aem.55.5.1279-1281.1989 | DOI Listing |
Sci Rep
January 2025
Department of Biotechnology, COMSATS University Islamabad, Abbottabad, Pakistan.
Malachite green (MG) is used as a dye for materials such as wood, cotton, and nylon, and is used in aquaculture to prevent fungal and protozoan diseases. However, it is highly toxic, with carcinogenic, mutagenic, and teratogenic properties, resulting in bans worldwide. Despite this, MG is still frequently used in many countries due to its efficacy and economy.
View Article and Find Full Text PDFAnal Chem
January 2025
Department of Atomic and Molecular Physics, Manipal Academy of Higher Education, Manipal 576104, India.
Slippery liquid-infused porous surfaces (SLIPSs) are a class of surface that offers low contact angle hysteresis and low tilt angle for water droplet shedding. This property also endows the surface with pinning-free evaporation, which in turn has been exploited for analyte concentration enrichment for Surface Enhanced Raman Spectroscopic applications and antibiofouling. Herein, we demonstrate a facile approach for creating SLIPS with low contact angle hysteresis and low tilt angle for water shedding by coating the equal-volume mixture of polydimethylsiloxane (PDMS) and silicone oil.
View Article and Find Full Text PDFProtein Sci
February 2025
Yusuf Hamied Department of Chemistry, University of Cambridge, Cambridge, UK.
We have recently demonstrated a novel anaerobic NADH-dependent haem breakdown reaction, which is carried out by a range of haemoproteins. The Yersinia enterocolitica protein, HemS, is the focus of further research presented in the current paper. Using conventional experimental methods, bioinformatics, and energy landscape theory (ELT), we provide new insight into the mechanism of the novel breakdown process.
View Article and Find Full Text PDFGut Microbes
December 2025
Department of Urology, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.
Hyperoxaluria, including primary and secondary hyperoxaluria, is a disorder characterized by increased urinary oxalate excretion and could lead to recurrent calcium oxalate kidney stones, nephrocalcinosis and eventually end stage renal disease. For secondary hyperoxaluria, high dietary oxalate (HDOx) or its precursors intake is a key reason. Recently, accumulated studies highlight the important role of gut microbiota in the regulation of oxalate homeostasis.
View Article and Find Full Text PDFCurr Med Chem
January 2025
Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Vietnam.
With the escalation of viral infections in recent decades, including the COVID- 19 pandemic, viral infectious diseases have increasingly become a global concern, attracting significant attention. Among many viral epidemics, the dengue virus, an RNA virus from the Flaviviridae family, has been reported by the WHO as one of the most prevalent mosquito-borne diseases, infecting roughly 400 million people yearly and spreading across all continents worldwide. In the last two decades, researchers from academia and industry have diligently studied many aspects of the virus, including its structure, life cycle, potential therapeutic agents, and vaccines.
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