Coculture of a sulfate-reducing bacterium, when grown in the absence of added sulfate, with Methanobacterium bryantii, which uses only H(2) and CO(2) for methanogenesis, degraded formate to CH(4). A pure culture of Desulfovibrio vulgaris JJ was able to produce small amounts of H(2). Such a syntrophic relationship might provide an additional way to avoid formate accumulation in anaerobic environments.
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http://dx.doi.org/10.1128/aem.52.6.1436-1437.1986 | DOI Listing |
Front Fungal Biol
July 2023
Department of Chemical Engineering, University of California, Santa Barbara, Santa Barbara, CA, United States.
Anaerobic fungi produce biomass-degrading enzymes and natural products that are important to harness for several biotechnology applications. Although progress has been made in the development of methods for extracting nucleic acids for genomic and transcriptomic sequencing of these fungi, most studies are limited in that they do not sample multiple fungal growth phases in batch culture. In this study, we establish a method to harvest RNA from fungal monocultures and fungal-methanogen co-cultures, and also determine an optimal time frame for high-quality RNA extraction from anaerobic fungi.
View Article and Find Full Text PDFCrit Rev Biotechnol
September 2024
NUS Environmental Research Institute, National University of Singapore, Singapore.
The generation of food waste (FW) is increasing at an alarming rate, contributing to a total of 32% of all the waste produced globally. Anaerobic digestion (AD) is an effective method for dealing with organic wastes of various compositions, like FW. Waste valorization into value-added products has increased due to the conversion of FW into biogas using AD technology.
View Article and Find Full Text PDFBiotechnol Bioeng
July 2023
Biodesign Swette Center for Environmental Biotechnology, Arizona State University, Tempe, Arizona, USA.
Hydrogen (H ) concentrations that were associated with microbiological respiratory processes (RPs) such as sulfate reduction and methanogenesis were quantified in continuous-flow systems (CFSs) (e.g., bioreactors, sediments).
View Article and Find Full Text PDFBiotechnol Biofuels
December 2021
Department of Chemical Engineering, University of California Santa Barbara, Santa Barbara, CA, 93106, USA.
ACS Synth Biol
September 2019
Department of Chemical Engineering , University of California Santa Barbara, Santa Barbara , California 93106 , United States.
Consortium-based approaches are a promising avenue toward efficient bioprocessing. However, many complex microbial interactions dictate community dynamics and stability that must be replicated in synthetic systems. The rumen and/or hindguts of large mammalian herbivores harbor complex communities of biomass-degrading fungi and bacteria, as well as archaea and protozoa that work collectively to degrade lignocellulose, yet the microbial interactions responsible for stability, resilience, and activity of the community remain largely uncharacterized.
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