AI Article Synopsis
- A new flotation method was created to examine soil and sediment microorganisms more effectively using polyvinylpyrrolidone instead of water.
- This technique allows for the extraction of gellike films from saturated soil samples, enabling quicker and more precise observation of the microbes under a light microscope after staining.
- Although only a small percentage (3.4 to 10.1%) of the total microbial population was recovered, it accurately represented the overall diversity and spatial relationships of the microorganisms present.
Article Abstract
An improved flotation method for microscopy of in situ soil and sediment microorganisms was developed. Microbial cells were released into gellike flotation films that were stripped from soil and sediment aggregates as these aggregates were submerged in 0.5% solutions of polyvinylpyrrolidone. The use of polyvinylpyrrolidone solutions instead of water facilitated the release of films from saturated samples such as aquifer sediments as well as from typical surface soils. In situ microbial morphological characteristics could then be surveyed rapidly by light microscopy of films stained with acridine orange. This method effectively determined the ranges of morphological diversity in a variety of sample types. It also detected microcolonies and other spatial relationships among microbial cells. Only a small fraction (3.4 to 10.1%) of the microflora was released into the flotation films, but plating and direct evaluations by microscopy showed that this fraction was representative of the total population.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC238902 | PMC |
| http://dx.doi.org/10.1128/aem.51.3.462-468.1986 | DOI Listing |
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