Incubated sediment slurries from Big Soda Lake, Nevada, produced significant levels of CH(4), and production was inhibited by 2-bromoethanesulfonic acid and by autoclaving. Methane production was stimulated by methanol, trimethylamine, and, to a lesser extent, methionine. Surprisingly, hydrogen, acetate, and formate amendments provided only slight or no stimulation of methanogenesis. Methane production by sediment slurries had a pH optimum of 9.7. A methanol-grown enrichment culture containing a small, epifluorescent coccus as the predominant organism was recovered from sediments. The enrichment grew best when FeS or autoclaved sediment particles were included in the media, had a pH optimum of 9.7, and produced CH(4) from CH(3)OH. The methane formed by methanolgrown enrichment cultures was depleted in C by 72 to 77 per thousand relative to the methanol.
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http://dx.doi.org/10.1128/aem.43.2.462-468.1982 | DOI Listing |
Environ Microbiol
February 2024
Department of Biology, University of Oregon, Eugene, Oregon, USA.
Big Soda Lake, Nevada, is a multi-extreme meromictic lake, whose hypersaline hyperalkaline bottom waters feature permanent anoxia and high concentrations of arsenic, sulphide and ammonia. These properties make Big Soda Lake-and the adjacent Little Soda Lake-a fascinating system for exploring life's boundaries, discovering novel microbial taxa and identifying biotechnologically useful strains. To date, the taxonomic diversity and metabolic capabilities of microorganisms in this system remain largely unknown.
View Article and Find Full Text PDFFoods
August 2023
Food Technology and Innovation Research Center of Excellence, School of Agricultural Technology and Food Industry, Walailak University, Nakhon Si Thammarat 80160, Thailand.
Mackerel (), a tropical dark-fleshed fish, has the potential to be used in the production of surimi. It is necessary to identify the optimal washing method to make better use of this species since efficient washing is the most important step in surimi processing to ensure maximal gelling and high-quality surimi. The purpose of this study was to evaluate the combined effect of cold carbonated water (CW) with NaCl and antioxidants in washing media, so-called antioxidant-infused soda-saline solution, on lipid and myoglobin removal efficacy, biochemical characteristics, gelling properties, sensory features, and the oxidative stability of mackerel surimi in comparison with unwashed mince (T1) and conventional water washed surimi (T2).
View Article and Find Full Text PDFJ Appl Microbiol
June 2023
Research Division of Environment and Health, Leibniz-Institute for Plasma Science and Technology e.V., Greifswald 17489, Germany.
Aim: The aim of the current study is to elucidate the inactivation and molecular response pattern of sublethal Listeria monocytogenes to cold plasma-mediated two-pronged oxidative microenvironments from a high-throughput multi-omics perspective.
Methods And Results: First joint transcriptomics and metabolomics analyses revealed that significantly expressed genes and metabolites were mainly involved in enhanced transmembrane transport and Fe2+/Cu+ efflux, amino acid limitation, cytoplasmic pH homeostasis, reconfiguration of central carbon metabolism flux, and energy conservation strategy, which triggered the surge of intracellular endogenous oxidative stress and finally mediated bacterial ferroptosis and pathogenicity attenuation. Typical antioxidant systems such as the TrxR-Trx system and common antioxidant genes (e.
Front Pediatr
September 2022
Department of Digital Anti-aging Healthcare (BK21), Graduate School of Inje University, Gimhae, South Korea.
Objective: This study identified factors related to adolescent obesity during the COVID-19 pandemic by using machine learning techniques and developed a model for predicting high-risk obesity groups among South Korean adolescents based on the result.
Materials And Methods: This study analyzed 50,858 subjects (male: 26,535 subjects, and female: 24,323 subjects) between 12 and 18 years old. Outcome variables were classified into two classes (normal or obesity) based on body mass index (BMI).
Health Promot Int
October 2022
Departamento de Nutrição, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
The Big Soda segment is central in modern food systems, and they invest heavily in digital marketing, but little is known about it. We aimed to analyze the digital marketing on Facebook of the soda brand with a major worldwide reach. Countries were described in terms of soda brand presence and popularity on Facebook according to countries' socio-demographic index (SDI) and the market share of soda brand (%MS).
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