Methanogenesis in big soda lake, nevada: an alkaline, moderately hypersaline desert lake.

Appl Environ Microbiol

U.S. Geological Survey, Menlo Park, California 94025, and Ames Research Center, National Aeronautics and Space Administration, Moffett Field, California 94035.

Published: February 1982

AI Article Synopsis

  • Incubated sediment slurries from Big Soda Lake showed significant methane (CH4) production, which was inhibited by certain chemicals and autoclaving.
  • Methane production was stimulated by compounds like methanol and trimethylamine, while hydrogen and acetate had little to no effect.
  • The optimal pH for methane production was found to be 9.7, and a culture enriched with methanol yielded a predominant organism that thrived best with specific additives, producing methane that was notably depleted in carbon compared to the original methanol source.

Article Abstract

Incubated sediment slurries from Big Soda Lake, Nevada, produced significant levels of CH(4), and production was inhibited by 2-bromoethanesulfonic acid and by autoclaving. Methane production was stimulated by methanol, trimethylamine, and, to a lesser extent, methionine. Surprisingly, hydrogen, acetate, and formate amendments provided only slight or no stimulation of methanogenesis. Methane production by sediment slurries had a pH optimum of 9.7. A methanol-grown enrichment culture containing a small, epifluorescent coccus as the predominant organism was recovered from sediments. The enrichment grew best when FeS or autoclaved sediment particles were included in the media, had a pH optimum of 9.7, and produced CH(4) from CH(3)OH. The methane formed by methanolgrown enrichment cultures was depleted in C by 72 to 77 per thousand relative to the methanol.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC241849PMC
http://dx.doi.org/10.1128/aem.43.2.462-468.1982DOI Listing

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