Nocardia amarae grown in a liquid medium induced coalescence of emulsions which differed in type, composition of the organic phase, and structure of stabilizing emulsifiers. De-emulsifying activity varied with the type of growth medium, culture age, and postharvest treatment. Based on extraction and degradation studies, it was concluded that de-emulsifying properties are due to the bacterial cell surface. Thus, bacteria may provide a new source of de-emulsifying agents.
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http://dx.doi.org/10.1128/aem.43.2.362-366.1982 | DOI Listing |
Rapid Commun Mass Spectrom
December 2024
Biomolecular Research Centre, Sheffield Hallam University, Sheffield, UK.
Rationale: Mycobacterial species contain high concentrations of mycolic acids in their cell wall. Mycobacteria can pose a threat to both human health and the environment. Mass spectrometry lipidomic characterization can identify bacterial species and suggest targets for microbiological interventions.
View Article and Find Full Text PDFInt J Syst Evol Microbiol
October 2022
Departamento de Microbiología y Genética, Campus Miguel de Unamuno, University of Salamanca, 37007 Salamanca, Spain.
The taxonomic status of two strains, designated BEN371 and CON9, isolated from stable foams on activated sludge plants was the subject of a polyphasic study which also included the type strains of species and three authenticated strains recovered from such foams. Phylogenetic analyses of 16S rRNA gene sequences showed that these isolates formed a compact cluster suggesting a well-supported lineage together with a second branch containing the strains. A phylogenomic tree based on sequences of 92 core genes extracted from whole genome sequences of the isolates, the strains and type strains confirmed the assignment of the isolates and the strains to separate but closely associated lineages.
View Article and Find Full Text PDFLett Appl Microbiol
October 2022
Department of Microbiology, Anatomy, Physiology and Pharmacology, La Trobe University, Bundoora, Vic, Australia.
The production of a stable foam on the surfaces of reactors is a global operating problem in activated sludge plants. In many cases, these foams are stabilized by hydrophobic members of the Mycolata, a group of Actinobacteria whose outer membranes contain long-chain hydroxylated mycolic acids. There is currently no single strategy which works for all foams.
View Article and Find Full Text PDFNat Microbiol
June 2021
Department of Physiology, Anatomy, and Microbiology, La Trobe University, Melbourne, Victoria, Australia.
Many wastewater treatment plants around the world suffer from the operational problem of foaming. This is characterized by a persistent stable foam that forms on the aeration basin, which reduces effluent quality. The foam is often stabilized by a highly hydrophobic group of Actinobacteria known as the Mycolata.
View Article and Find Full Text PDFJ Mol Microbiol Biotechnol
April 2021
Department of Civil and Environmental Engineering, University of California, Irvine, California, USA.
An existing bead-beating DNA extraction protocol was employed to compare the DNA extraction recovery and fragment quality of 6 different aeration diffuser biofilms. Escherichia coli, Gordonia amarae, and mixed liquor were used as controls. The fraction of total DNAbiofilm decreased monotonically with increasing number of beat beatings (BB) when the amount of DNA present was sufficient (>4 μgDNA/cm2), excluding the ceramic disk.
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