Lignin biodegradation in a variety of natural materials was examined using specifically labeled synthetic C-lignins. Natural materials included soils, sediments, silage, steer bedding, and rumen contents. Both aerobic and anaerobic incubations were used. No C-labeled lignin biodegradation to labeled gaseous products under anaerobic conditions was observed. Aerobic C-labeled lignin mineralization varied with respect to type of natural material used, site, soil type and horizon, and temperature. The greatest observed degradation occurred in a soil from Yellowstone National Park and amounted to over 42% conversion of total radioactivity to CO(2) during 78 days of incubation. Amounts of C-labeled lignin mineralization in Wisconsin soils and sediments were significantly correlated with organic carbon, organic nitrogen, nitrate nitrogen, exchangeable calcium, and exchangeable potassium.
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http://dx.doi.org/10.1128/aem.33.1.43-51.1977 | DOI Listing |
Nat Commun
January 2025
Institute of Marine Science and Technology, Shandong University, Qingdao, China.
Lignin, as the abundant carbon polymer, is essential for carbon cycle and biorefinery. Microorganisms interact to form communities for lignin biodegradation, yet it is a challenge to understand such complex interactions. Here, we develop a coastal lignin-degrading bacterial consortium (LD), through "top-down" enrichment.
View Article and Find Full Text PDFNPJ Syst Biol Appl
January 2025
The Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, CA, 94608, USA.
Genome-scale metabolic models (GSMM) are commonly used to identify gene deletion sets that result in growth coupling and pairing product formation with substrate utilization and can improve strain performance beyond levels typically accessible using traditional strain engineering approaches. However, sustainable feedstocks pose a challenge due to incomplete high-resolution metabolic data for non-canonical carbon sources required to curate GSMM and identify implementable designs. Here we address a four-gene deletion design in the Pseudomonas putida KT2440 strain for the lignin-derived non-sugar carbon source, p-coumarate (p-CA), that proved challenging to implement.
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
Science and Technology Center for Sustainability (CCTS), Federal University of São Carlos (UFSCar), João Leme dos Santos, km 110, 18052-780 Sorocaba, SP, Brazil. Electronic address:
The growing demand for sustainable solutions in agriculture, driven by global population growth and increasing soil degradation, has intensified the search for sustainable soil conditioners. This study investigated the impact of adding nanoclay (NC) and nano lignin (NL) to thermoplastic starch (TPS) on its physical, chemical, and thermal properties, its effectiveness as a soil conditioner, and its resistance to UV-C degradation. TPS nanocomposites were prepared with varying NC (3 %, 5 %, 7 %) and NL (0.
View Article and Find Full Text PDFFood Chem (Oxf)
June 2025
College of Biology and Environment, Zhejiang Wanli University, No. 8 Qianhu South Road, Ningbo 315000, China.
Grapes are prone to softening, which limits their shelf life and suitability for long-distance transport. This study explored the molecular mechanisms underlying the effects of the chemical preservatives gibberellin (GA) and the nitric oxide donor sodium nitroprusside (SNP) on grape firmness. Enhancing grape quality, prolonging shelf life, and extending market supply were key objectives.
View Article and Find Full Text PDFMicrobiome
January 2025
Instituto de Investigación de La Viña y El Vino, Escuela de Ingeniería Agraria, Universidad de León, Avenida de Portugal, 41, León, 24009, Spain.
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