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Reproducibility and Consistency of Isolation Protocols for Fibroblasts, Smooth Muscle Cells, and Epithelial Cells from the Human Vagina.

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January 2025

Reproductive Biology Laboratory, Amsterdam UMC-Location AMC, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands.

(1) Background: For the reconstruction of a human vagina, various surgical procedures are available that are often associated with complications due to their failure to mimic the physiology of the human vagina. We recently developed a vascularized, organ-specific matrix from healthy human vaginal wall tissue with suitable biomechanical properties. A superior graft would require further extensive colonization with autologous vaginal cells to reduce complications upon implantation.

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Mayer-Rokitansky-Küster-Hauser (MRKH) syndrome is a disorder marked by the congenital absence of the uterus and vagina. Patients with this condition often present with primary amenorrhoea and normal secondary sexual characteristics. The diagnosis of MRKH syndrome has profound implications for a patient's fertility and psychological well-being, necessitating a multidisciplinary approach that includes psychosocial support.

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There are a variety of surgical conditions impacting the canine vagina and vestibule that require access through a caudal approach. A standard vertical episiotomy involves making an incision beginning ventral to the anus and extending ventrally through the dorsal commissure of the vulva and into the lumen of the vestibule and distal vagina. The objective of this study was to determine if complex vaginal procedures could be performed via a transverse perineal approach, thus eliminating the need to incise and reconstruct the vulva as performed in a standard episiotomy, and to evaluate the feasibility of vaginal resection with vulvar-sparing vestibular urethrostomy using this transverse approach.

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Onabotulinum Toxin A-Led Urinary Tract Infections-Should we Safeguard? A Randomized Controlled Trial.

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Department of Urogynecology and Reconstructive Pelvic Surgery, Atlantic Health System, 435 South Street, Suite 370, Morristown, NJ, 07960, USA.

Introduction And Hypothesis: The objective of our study was to evaluate the need for antibiotic prophylaxis for urinary tract infection (UTI) prevention before Onabotulinum toxin A injection for overactive bladder (OAB). We hypothesize that the lack of antibiotic prophylaxis might not be inferior to administering prophylaxis.

Methods: This was a multi-centered, nonblinded, randomized controlled trial conducted between August 2022 and September 2024.

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