Objective: To study the chemical constituents of the leaf of Isatis indigotica.
Method: Chromatography and spectral analysis were respectively used to isolate and identify the constituents.
Result: Three compounds were isolated from the ethanol extracts of theleaf of I. indigotica, and identified as indirubin, tryptanthrin and L-pyroglutamic acid.
Conclusion: L-pyroglutamic acid was isolated from the genus for the first time, and tryptanthrin was isolated from the leaf of this plant for the first time.
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