We evaluated the Roche LightCycler Staphylococcus M(GRADE) kits to differentiate between Staphylococcus aureus and coagulase-negative staphylococci in blood cultures growing clusters of gram-positive cocci. Testing 100 bottles (36 containing S. aureus), the assay was 100% sensitive and 98.44% specific for S. aureus and 100% sensitive and specific for coagulase-negative staphylococci.
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1317217 | PMC |
http://dx.doi.org/10.1128/JCM.43.12.6144-6146.2005 | DOI Listing |
J Microbiol Methods
March 2023
Public Health General Directorate, Department of Microbiology Reference Laboratories and Biological Products, Ankara, Turkey.
Aims: A new multiplex real-time PCR (qPCR) assay was developed to detect antibiotic-resistant hospital-acquired infectious agents in nasal and rectal swab samples in 1.5 h without the need for nucleic acid extraction.
Methods: Spiked negative clinical specimens were used for the analytical performance evaluation.
Infect Drug Resist
August 2021
Facultad de Ingenierías y Ciencias Aplicadas, Biotecnología, Universidad de Las Américas, Quito, Ecuador.
Background: Methicillin-resistant (MRSA) is resistant to most of the commonly used antibiotics and is therefore a public health issue. Colonization with MRSA is a risk factor for infection or transmission.
Purpose: To determine the prevalence of colonization with (SA) and MRSA strains in health care workers (HCWs) at a tertiary hospital in Ecuador and to determine the risk factors associated with carriage.
J Burn Care Res
November 2020
Department of Thoracic Surgery, Qom University of Medical Sciences, Iran.
Methicillin-resistant Staphylococcus aureus is the cause of nosocomial and community-acquired infections. This study aimed to evaluate the effect of zinc oxide and silver nanoparticles (ZnO-Ag NPs) on biofilms formation and icaA gene expression in methicillin-resistant S. aureus (MRSA).
View Article and Find Full Text PDFPDA J Pharm Sci Technol
April 2019
Biology and Horticulture Department, Bergen Community College, Paramus, NJ.
A real-time polymerase chain reaction (RT-PCR) assay was developed to detect in pharmaceutical products contaminated with low levels of bacteria. Different pharmaceutical suspensions were artificially contaminated with , , , and After a 24 h incubation in trypticase soy broth with Tween 20, samples were streaked on mannitol salt, phenyl ethyl alcohol, eosin methylene blue, MacConkey, and pseudomonas isolation agar. Microbial DNA was extracted from each sample by using a Tris-EDTA, proteinase K, Tween 20 buffer.
View Article and Find Full Text PDFMod Rheumatol
November 2017
a Department of Orthopedic Surgery , Yokohama City University, Yokohama , Japan.
Objectives: A new multiplex real-time polymerase chain reaction (PCR) assay was developed to detect methicillin-resistant Staphylococcus (MRS) and to distinguish between gram-positive and gram-negative bacteria. In this study, we validated the sensitivity and specificity of this assay with periprosthetic joint infections (PJIs) and evaluated the utility of PCR for culture-negative PJI.
Methods: Forty-five samples from 23 infectious PJI cases and 106 samples from 64 non-infectious control cases were analyzed by real-time PCR using a LightCycler Nano system.
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!