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Collection and Processing of Samples for Next-Generation Sequencing to Study the Gut Microbiome.
Methods Mol Biol
November 2024
Department of Biomedical Sciences, Cooper Medical School of Rowan University, Camden, NJ, USA.
16S rRNA gene sequencing is commonly used for identification and quantitation of microorganisms in complex biological mixtures, such as the human gut microbiome. The 16S rRNA gene is an excellent target gene for sequencing DNA in a heterogenous sample, as it is a highly conserved part of the transcriptional machinery. Universal PCR primers are used to amplify the conserved regions of 16S.
View Article and Find Full Text PDFA Systematic Review of Quantitative Methods for Evaluating Electronic Medication Administration Record and Bar-Coded Medication Administration Usability.
Appl Clin Inform
January 2023
National Center for Human Factors in Healthcare, MedStar Health Research Institute, Washington, District of Columbia, United States.
Article Synopsis
- eMARs and BCMA technologies have improved medication safety, but their poor usability can pose safety risks.
- This systematic review aimed to evaluate how the design of eMAR and BCMA affects usability in terms of efficiency, effectiveness, and satisfaction.
- Out of 1,922 articles, 41 were analyzed, revealing that BCMA and eMAR implementations significantly improved effectiveness and satisfaction but showed less impact on efficiency, indicating a need for future research to enhance efficiency in eMAR systems.
Clonal hematopoiesis in the donor does not adversely affect long-term outcomes following allogeneic hematopoietic stem cell transplantation: result from a 13-year follow-up.
Haematologica
July 2023
Division of Medical Oncology and Hematology, Princess Margaret Cancer Centre, Toronto, Canada; Institute for Medical Science, Faculty of Medicine, University of Toronto, Toronto.
Donor clonal hematopoiesis may be transferred to the recipient through allogeneic hematopoietic stem cell transplantation (HSCT), but the potential for adverse long-term impact on transplant outcomes remains unknown. A total of 744 samples from 372 recipients who received HSCT and the corresponding donors were included. Bar-coded error-corrected sequencing using a modified molecular inversion probe capture protocol was performed, which targeted 33 genes covering mutations involved in clonal hematopoiesis with indeterminate potential (CHIP) and other acute myeloid leukemia-related mutations.
View Article and Find Full Text PDFscRNA-seq for Microcephaly Research [II]: Preparation of Single-Cell cDNA Libraries.
Methods Mol Biol
November 2022
Department of Neurology, University of North Carolina Medical School, Chapel Hill, NC, USA.
Droplet-based single-cell RNA-seq (scRNA-seq) requires the addition of bar codes that mark the cell and transcript of origin. Cell-specific bar codes, typically added by cDNA elongation on beads, label each transcript derived from an individual cell. Transcript-specific bar codes serve as unique molecular identifiers and allow for the maintenance of transcript proportions after PCR-based amplification of cDNA.
View Article and Find Full Text PDFEffects of Hericium erinaceus Hedgehog mushroom on the endophytic microbial community of the host plant.
J Basic Microbiol
January 2023
School of Life Sciences, Zhengzhou University, Zhengzhou, Henan, China.
Hedgehog mushroom is a Hericium erinaceus associated with fagaceae and pinaceae trees in the northern hemisphere. It is still unknown whether this symbiotic relationship will affect the endophytic microbial community of the host plants. In this study, the endophytic microbial communities of different Quercus aliena tissues (root, stem, and leaf) with or without H.
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