The first inducible Arthrobacter overexpression system, based on the promoter/operator and the repressor of the 6-D-hydroxynicotine oxidase gene of Arthrobacter nicotinovorans, is described here. Nicotine-dependent overproduction and affinity purification of recombinant proteins are presented. The system will allow the production of complex enzymes and genetic complementation studies in Arthrobacter species.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1317448 | PMC |
| http://dx.doi.org/10.1128/AEM.71.12.8920-8924.2005 | DOI Listing |
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