AI Article Synopsis
- The study examined haloarchaeal diversity in the Secovlje salterns using 16S rRNA and bacteriorhodopsin gene fragments, finding a higher diversity than previously noted in hypersaline environments.
- The analysis of 180 clones revealed 15 unique 16S rRNA and 10 bacteriorhodopsin phylotypes, with rarefaction analysis suggesting even more diversity could be uncovered with further sampling.
- Most sequences were clustered within the Halorubrum group, while the expected dominant 'Haloquadratum' relatives were found to be rare, indicating a unique haloarchaeal community likely influenced by the saltern's unique salt production processes.
Article Abstract
Haloarchaeal diversity in the crystallizers of Adriatic Secovlje salterns was investigated using gene fragments encoding 16S rRNA and bacteriorhodopsin as molecular markers. Screening of 180 clones from five gene libraries constructed for each gene targeted revealed 15 different 16S rRNA and 10 different bacteriorhodopsin phylotypes, indicating higher haloarchaeal diversity than previously reported in such hypersaline environments. Furthermore, results of rarefaction analysis indicated that analysis of an increasing number of clones would have revealed additional diversity. Finally, most sequences from the crystallizers grouped within the Halorubrum branch, whereas square-shaped 'Haloquadratum' relatives, repeatedly reported to dominate crystallizer communities, were rare. Presence of such special and diverse haloarchaeal community could be attributed to the Secovlje salterns rare continuous short-cycling salt production mechanism.
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| http://dx.doi.org/10.1016/j.femsec.2005.06.004 | DOI Listing |
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Article Synopsis
- The study examined haloarchaeal diversity in the Secovlje salterns using 16S rRNA and bacteriorhodopsin gene fragments, finding a higher diversity than previously noted in hypersaline environments.
- The analysis of 180 clones revealed 15 unique 16S rRNA and 10 bacteriorhodopsin phylotypes, with rarefaction analysis suggesting even more diversity could be uncovered with further sampling.
- Most sequences were clustered within the Halorubrum group, while the expected dominant 'Haloquadratum' relatives were found to be rare, indicating a unique haloarchaeal community likely influenced by the saltern's unique salt production processes.
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