At least three major antigenic dengue 2 virus proteins were recognized by pooled dengue fever patients' sera in infected Aedes albopictus (C6/36) mosquito cells. Dengue virus envelope (E), premembrane (PrM) and non-structural protein 1 (NS 1) dimer were detected beginning on day 3 postinfection in both the cell membrane and cytosolic fractions. Using the patients' sera, the presence of antigenic intermediate core protein (C)-PrM and NS1-non-structural protein 2a (NS2a) in the cytoplasmic fraction of dengue 2 virus infected cells was revealed. The presence of a approximately 92 and approximately 84 kDa NS 1 dimer in the membrane (NS 1m) and cytosolic (NS 1c) fractions of C6/36 cells, respectively, was also recognized. Using individual patient's serum, it was further confirmed that all patients' sera contained antibodies that specifically recognized E, NS 1 and PrM present in the dengue 2 virus-infected cell membrane fractions, suggesting that these glycosylated virus proteins were the main antigenic proteins recognized in vivo. Detection of dengue 2 virus C antibody in some patients further suggested that C could be antigenic if presented in vivo.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Blood meal analysis: unveiling the feeding preferences of Aedes aegypti and Aedes albopictus in a dengue-endemic area.
Trop Biomed
December 2024
Department of Parasitology, Faculty of Medicine, Center of Insect Vector Study, Chiang Mai University, Chiang Mai, Thailand.
Studies have suggested animals as possible reservoir hosts for flaviviruses transmitted by Aedes mosquitoes; however, there is limited evidence for the dengue virus in Malaysia. One of the possible ways to determine the zoonotic potential for any pathogen transmission is through blood meal analysis which can provide valuable insights into the feeding preferences of the mosquitoes. Unfortunately, limited information is available on the feeding preferences of Aedes mosquitoes in Malaysia.
View Article and Find Full Text PDFAssociation of total and dengue-specific IgE levels in the sera with dengue virus inhibition and antibody-dependent enhancement.
Trop Biomed
December 2024
Department of Medical Microbiology, Faculty of Medicine, Universiti Malaya, 50603 Kuala Lumpur, Malaysia.
Dengue, caused by the dengue virus (DENV), poses a significant global health challenge. Effective vaccines and treatments for dengue are lacking due to gaps in understanding its pathogenesis and mechanisms in severe cases. This study investigates the role of immunoglobulin E (IgE) in dengue, focusing on its potential association with virus neutralization and antibody-dependent enhancement (ADE) in DENV replication.
View Article and Find Full Text PDFDynamic trends of dengue fever serotypes in northern India: Exploring clinical manifestations, serotype dissemination, and the influence of mixed infections.
Trop Biomed
December 2024
Department of Microbiology, ESIC Medical College & Hospital, Faridabad, 121001, Haryana, India.
Examining the co-circulation of various serotypes and finding serotypes linked to illness severity were the main objectives of this study, which sought to investigate the epidemiology and serotype distribution of dengue in Haryana, North India. The cross-sectional study, which was carried out in a tertiary care hospital between September 2021 and April 2023, enrolled participants who met WHO criteria for probable dengue fever. Blood samples underwent molecular and serological diagnostics, such as immunochromatographic testing, VIDAS® Dengue NS1 assays, and TRUPCR® Dengue Detection and serotyping kits, in addition to the collection of clinical and demographic data.
View Article and Find Full Text PDFEnhancing surveillance for dengue fever in Oyo State, Nigeria - a one health approach.
One Health Outlook
January 2025
Medical Virology Unit, Faculty of Basic Medical and Applied Sciences, Lead City University and Primary Health Care Board, Ibadan, Oyo State, Nigeria.
Background: Dengue fever (DF) poses a growing global threat, necessitating a comprehensive one-health approach to address its complex interplay between human, animal, and environmental factors. In Oyo State, Nigeria, the true burden of DF remains unknown due to underdiagnosis and misdiagnosis as malaria, exacerbated by poor health-seeking behavior, weak surveillance systems, and inadequate health infrastructure. Adopting a one-health approach is crucial to understanding the dynamics of DF transmission.
View Article and Find Full Text PDFEvaluation of Multipeptide Sequences Identified in Silico for the Serological Detection of Antibodies against Vector-Borne Diseases.
Am J Trop Med Hyg
January 2025
Centro de Investigaciones Regionales "Dr. Hideyo Noguchi," Universidad Autónoma de Yucatán, Mérida, México.
The socioecological conditions of Mexican regions are conducive to the spread of vector-borne diseases. Although there are established treatment guidelines for dengue and rickettsiosis, diagnosis is complicated. The objective of this work was to identify epitopes of Rickettsia and dengue virus that could be used in serology screening against vector-borne diseases.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!