Unlike those of the wild-type strain, proheterocysts of the Anabaena sp. strain PCC 7120 hetC strain keep dividing. ftsZ, the most critical cell division gene, is up-regulated in hetC proheterocysts. Heterocyst differentiation genes hglD, hglE, patB, nifB, and xisA are no longer expressed in the hetC mutant. hetC also regulates the expression of patA, a pattern formation gene.
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http://dx.doi.org/10.1128/JB.187.24.8489-8493.2005 | DOI Listing |
Chemosphere
January 2025
U.S. Environmental Protection Agency, Office of Research and Development, Cincinnati, OH, USA. Electronic address:
Cyanobacteria harmful algal blooms in lakes are primarily driven by nutrient and temperature conditions, yet the interplay of these abiotic factors with microbial community dynamics during bloom events is complex and challenging to unravel. Despite advances through deep sequencing approaches, the underlying transcriptomic changes occurring within blooming and non-blooming taxa remains an actively expanding area of study. In this work, we examined a spring-summer bloom event in Anderson Lake, WA, which has experienced recurring annual blooms dominated by the filamentous, anatoxin-a producing, diazotroph: Dolichospermum sp.
View Article and Find Full Text PDFNucleic Acids Res
December 2024
Genetics and Experimental Bioinformatics, Faculty of Biology, University of Freiburg, Schänzlestr. 1, 79104 Freiburg, Germany.
RNA-binding proteins (RBPs) are central components of gene regulatory networks. The differentiation of heterocysts in filamentous cyanobacteria is an example of cell differentiation in prokaryotes. Although multiple non-coding transcripts are involved in this process, no RBPs have been implicated thus far.
View Article and Find Full Text PDFMol Microbiol
January 2025
Instituto de Bioquímica Vegetal y Fotosíntesis, CSIC and Universidad de Sevilla, Seville, Spain.
Cyanobacteria developed oxygenic photosynthesis and represent the phylogenetic ancestors of chloroplasts. The model strain Anabaena sp. strain PCC 7120 grows as filaments of communicating cells and can form heterocysts, cells specialized for N fixation.
View Article and Find Full Text PDFHarmful Algae
November 2024
Key Laboratory of Lake and Watershed Science for Water Security, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China; State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China; University of Chinese Academy of Sciences, Nanjing 211135, China. Electronic address:
In recent years, initiatives aimed at mitigating eutrophication have successfully reduced nitrogen and phosphorus concentrations in numerous lakes across China. Notably, the management of total nitrogen levels has prompted a shift in the dominant genera responsible for harmful algal blooms. Among these, Dolichospermum, a typical diazotrophic cyanobacterium, exhibits the ability to differentiate heterocysts for atmospheric N fixation under nitrogen-limited conditions.
View Article and Find Full Text PDFMicrobiol Res
January 2025
State Key Laboratory of Freshwater Ecology and Biotechnology, Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430070, Hubei, China; Hubei Hongshan Laboratory, Wuhan 430070, Hubei, China. Electronic address:
PacR (All3953) has previously been identified as a global transcriptional regulator of carbon assimilation in cyanobacteria. In the facultative diazotrophic and filamentous cyanobacterium Anabaena PCC 7120 (Anabaena), inactivation of pacR has been shown to affect cell growth under various conditions. Nitrogen fixation in Anabaena occurs in heterocysts, cells differentiated semiregularly along the filaments following deprivation of combined nitrogen such as nitrate or ammonium.
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