Objective: To synthesize highly pure HBV post-transcriptional regulatory element (HPRE) via transcription in vitro by T7 RNA polymerase.
Methods: HPRE gene was amplified by PCR from a template containing HBV complete genomic DNA and cloned into plasmid pGEM-11zf. The cloned DNA sequence was transcribed by T7 RNA polymerase.
Results: The construction of HPRE gene recombinant plasmid and production of HPRE via transcription in vitro was successful.
Conclusion: In vitro transcription by T7 RNA polymerase can be used to synthesize highly pure HPRE.
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