Trimethoprim, a widely used antimicrobial drug was tested for its effect on the level of nuclear DNA damage in cultured peripheral blood lymphocytes in terms of chromosome and DNA alterations. The extent of cytogenetic damage, expressed as chromosome breakage and chromosome loss, was evaluated employing the cytokinesis block micronucleus method (CBMN) in cultured peripheral blood lymphocytes coupled with fluorescence in situ hybridization (FISH) using a digoxigenin-labelled alphoid DNA probe specific for the centromere of all human chromosomes. The DNA breakage level was evaluated by the Comet assay. Cultures were set up by using blood samples from two healthy donors. A range of concentrations of the test agent (from 1 to 100 microg/ml) was used for the micronuclei (MN) frequency and cytogenetic origin of MN. For the Comet assay the range of doses used was from 0.5 to 150 microg/ml. From the results obtained it appears that this antifolic agent has a significant clastogenic potential, as detected by a dose-dependent increase of the incidence of C-MN and significantly greater than control levels at the highest concentrations tested (25,100 microg/ml). In addition, the results obtained in the Comet assay also show that trimethoprim induces a dose-dependent increase in the level of DNA breakage, this increase attaining statistical significance at the highest concentrations tested (25, 100, 150 microg/ml), which would confirm its genotoxicity.
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http://dx.doi.org/10.1016/j.tiv.2005.10.008 | DOI Listing |
Environ Res
January 2025
Doctorado en Ciencias Ambientales, Centro de Ciencias de Desarrollo Regional, Universidad Autónoma de Guerrero. Privada de Laurel 13, Col. El Roble, 39640, Acapulco, Guerrero, México; Facultad de Ciencias Agropecuarias y Ambientales, Unidad Tuxpan, Universidad Autónoma de Guerrero. Carretera Iguala-Tuxpan, km 2.5, Iguala de la Independencia, Guerrero, México; Facultad de Ciencias Agropecuarias, Universidad Autónoma del Estado de Morelos. Avenida Universidad 1001, 62210, Cuernavaca, Morelos, México; Laboratorio de Toxicología Ambiental, Departamento de Ciencias Ambientales, Instituto de Ciencias de la Atmósfera y Cambio Climático, Universidad Nacional Autónoma de México, Ciudad Universitaria Coyoacán, Ciudad de México 04510, México; Centro Nacional de Recursos Genéticos, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Tepatitlán de Morelos, 47600, Jalisco, México; Escuela Superior de Ciencias de la Tierra, Universidad Autónoma de Guerrero. Ex-hacienda de San Juan Bautista, Taxco el Viejo, 40323, Taxco el Viejo, Guerrero, México. Electronic address:
This study explored the distribution of macronutrients (Ca, Mg, Na, K) and lithogenic (Ba, Cr, Ni, Mn, Fe) and mining-related (As, Pb, Cd, Cu, Zn) toxic metalloids and metals (TMMs) in tomato (Solanum lycopersicum L.), and its effects on plant development, productivity, genotoxicity, and human health, using a soil affected by mine tailings (AS) and an unaffected control soil (CS). The chemistry of soils reflected their mineralogy, and Fe-Ti oxides, sulfides and sulfosalts were found to be the most significant reservoirs of TMMs.
View Article and Find Full Text PDFClin Exp Pharmacol Physiol
March 2025
Department of Endocrinology, The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin, People's Republic of China.
Isoferulic acid (IA), a derivative of cinnamic acid, is derived from Danshen and exhibits anticancer properties by disrupting cancer cell activities. However, its role in pancreatic cancer, the "king of cancer", was unknown. In this study, pancreatic cancer cells were subjected to treatment with IA (6.
View Article and Find Full Text PDFNutrients
January 2025
Center of Excellence Food Technology and Nutrition, University of Applied Sciences Upper Austria, Stelzhamerstraße 23, 4600 Wels, Austria.
Individuals with special metabolic demands are at risk of deficiencies in fat-soluble vitamins, which can be counteracted via supplementation. Here, we tested the ability of micellization alone or in combination with selected natural plant extracts to increase the intestinal absorption and bioefficacy of fat-soluble vitamins. Micellated and nonmicellated vitamins D3 (cholecalciferol), D2 (ergocalciferol), E (alpha tocopheryl acetate), and K2 (menaquionone-7) were tested in intestinal Caco-2 or buccal TR146 cells in combination with curcuma (), black pepper (), or ginger () plant extracts.
View Article and Find Full Text PDFPathogens
January 2025
Department of Pharmacology and Toxicology, Faculty of Biosciences, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan.
(1) Background: Surra is a debilitating disease of wild and domestic animals caused by (), resulting in significant mortality and production losses in the affected animals. This study is the first to assess the genetic relationships of in naturally affected buffaloes from Multan district, Pakistan, using ITS-1 primers and evaluating the effects of parasitemia and oxidative stress on DNA damage and hematobiochemical changes in infected buffaloes. (2) Methods: Blood samples were collected from 167 buffaloes using a multi-stage cluster sampling strategy, and trypomastigote identification was performed through microscopy and PCR targeting RoTat 1.
View Article and Find Full Text PDFMedicina (Kaunas)
December 2024
Department of Internal Medicine, Division of Endocrinology, University Hospital Centre Zagreb, Croatian Referral Center for Obesity Treatment, Kišpatićeva 12, 10000 Zagreb, Croatia.
The prevalence of metabolic syndrome (MetS) worldwide is rapid and significant on a global scale. A 2022 meta-analysis of data from 28 million individuals revealed a global prevalence of 45.1%, with notably higher rates in the Eastern Mediterranean Region and the Americas, particularly in high-income countries.
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