AI Article Synopsis
- The study investigates how oxidative stress, particularly hydrogen peroxide (H2O2), affects the growth and differentiation of L6 myoblasts.
- The results show that low concentrations of H2O2 can stimulate cell growth and increase the expression of myogenin, a key marker for muscle cell differentiation.
- The findings suggest that reactive oxygen species can act as signaling molecules to promote the development and maturation of myoblasts into myotubes.
Article Abstract
Objective: To explore the relationship between the differentiation of L6 myoblasts and oxidative stress.
Methods: MTT assay was used to determine the viability of L6 myoblasts, from which the total RNA was extracted for amplification of the myogenin gene fragment by RT-PCR. H(2)O(2)-induced morphological changes of the cells were observed.
Results: The myoblasts treated with low concentration of reactive oxygen (50 micromol/L H(2)O(2)) for 1 h exhibited accelerated cell growth (P<0.05), and treatment with 50 and 150 micromol/L H(2)O(2) induced the gene expression of myogenin, a molecular marker for differentiation of myoblasts. Morphological study revealed myotube formation and accelerated differentiation of the myoblasts induced by H(2)O(2).
Conclusion: The reactive oxygen may serve as the intracellular signal molecules to induce the growth and differentiation of the myoblasts.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Molecular aspects of cytoprotection by Optineurin during stress and disease.
Biochim Biophys Acta Mol Cell Res
January 2025
CSIR-Centre for Cellular and Molecular Biology, Hyderabad 500007, India; Florida Research and Innovation Center, Cleveland Clinic, Port Saint Lucie, FL 34987, USA. Electronic address:
Optineurin/OPTN is an adapter protein that plays a crucial role in mediating many cellular functions, including autophagy, vesicle trafficking, and various signalling pathways. Mutations of OPTN are linked with neurodegenerative disorders, glaucoma, and amyotrophic lateral sclerosis (ALS). Recent work has shown that OPTN provides cytoprotection from many types of stress, including oxidative stress, endoplasmic reticulum stress, protein homeostasis stress, tumour necrosis factor α, and microbial infection.
View Article and Find Full Text PDFFerristatin II protects nucleus pulposus against degeneration through inhibiting ferroptosis and activating HIF-1α pathway mediated mitophagy.
Int Immunopharmacol
January 2025
Department of Spine Surgery, Shandong Provincial Hospital affiliated to Shandong First Medical University, Jinan, Shandong 250000, China; Department of Spine Surgery, Shandong Provincial Hospital, Shandong University, Jinan, Shandong 250000, China. Electronic address:
Background: Nucleus pulposus (NP) degeneration represents a significant contributing factor in the pathogenesis of intervertebral disc (IVD) degeneration (IVDD), and is a key underlying mechanism in several lumbar spine pathologies. Nevertheless, the precise mechanisms that govern NP degeneration remain unclear. A significant contributing factor to IVDD has been identified as ferroptosis.
View Article and Find Full Text PDFTau phosphorylation suppresses oxidative stress-induced mitophagy via FKBP8 receptor modulation.
PLoS One
January 2025
Department of Anesthesiology & Perioperative Medicine, University of Rochester, Rochester, New York, United States of America.
Neurodegenerative diseases are often characterized by mitochondrial dysfunction. In Alzheimer's disease, abnormal tau phosphorylation disrupts mitophagy, a quality control process through which damaged organelles are selectively removed from the mitochondrial network. The precise mechanism through which this occurs remains unclear.
View Article and Find Full Text PDFComparison of oxidative stress status in the kidney tissue of male rats treated with paraquat and nanoparaquat.
Sci Rep
January 2025
Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
The study aimed to compare the oxidative stress status in the kidney tissue of rats treated with paraquat and nanoparaquat. The levels of oxidative stress markers, including malondialdehyde (MDA), total antioxidant capacity (TAC), and thiol groups (TTG), were measured in the kidney tissue samples. A total of forty male Wistar rats were randomly assigned to eight groups, each consisting of five rats: a control group, a paraquat (PQ) group, an N-acetylcysteine (NAC) group, groups receiving nanoparaquat α and β (α and β), groups receiving PQ and NAC (PQ + NAC), and groups receiving nanoparaquat α and β with NAC (+ NACα and β).
View Article and Find Full Text PDFMANF overexpression ameliorates oxidative stress-induced apoptosis of human nucleus pulposus cells by facilitating mitophagy through promoting MFN2 expression.
Sci Rep
January 2025
Department of Spine Surgery, The First Affiliated Hospital of Xinjiang Medical University, No.137, Liyu Mountain South Road, Urumqi City, 830054, Xinjiang Province, China.
Intervertebral disc degeneration (IDD) is a degenerative condition associated with impaired mitophagy. MANF has been shown to promote mitophagy in murine kidneys; however, its role in IDD remains unexplored. This study aimed to elucidate the mechanism by which MANF influences IDD development through the regulation of mitophagy.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!