Expressed protein ligation to study protein interactions: semi-synthesis of the G-protein alpha subunit.

Protein Pept Lett

Department of Biochemistry and Molecular Biophysics, Washington University, St. Louis, MO 63110, USA.

Published: November 2005

Interactions between G proteins and GPCRs are fundamental for transmitting signals for a multitude of physiologic responses. Little is known regarding the protein-protein interface between the G protein and the receptor, much less the mechanisms for receptor activation of G proteins. Here, we will describe how expressed protein ligation will aid in the study of protein-protein interactions between semi-synthetic G alpha subunits and GPCRs.

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http://dx.doi.org/10.2174/0929866054864175DOI Listing

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