Conclusion: The results of this study demonstrate that suppression of inflammation by dexamethasone attenuates the host immune response against adenoviral-mediated gene transfection and thereby prolongs transgene expression in murine nasal mucosa.
Objectives: Gene transfer using a recombinant adenovirus is a good tool for research and clinical applications, but the immune response to adenoviral vectors can induce inflammation and loss of transgene expression in transfected tissues. In this study we investigated the effects of dexamethasone-induced immunosuppression on adenovirus gene transfer in the nasal mucosa of the mouse.
Material And Methods: We administered the recombinant adenovirus Ax1CAlacZ, which encodes Escherichia coli beta-galactosidase (lacZ gene), to the nasal mucosa of mice treated with or without i.p. dexamethasone and evaluated the expression of the lacZ gene on Days 2, 4, 7, 14 and 28. The nasal mucosa was dissected out, and the mRNA level was measured using a LightCycler. The expression of the exogenous beta-galactosidase was detected by means of histochemistry.
Results: Dexamethasone treatment significantly increased the mRNA level compared with that in the controls at Days 4, 7 and 14. Histochemistry showed that the expression of beta-galactosidase protein persisted in the dexamethasone-treated mice at Days 7 and 14 but had diminished almost to nothing in the control group.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1080/00016480410018160 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Background: Abnormal protein depositions of amyloid β and tau are present in the nasal cavity in patients with Alzheimer's disease. This finding raises an idea that nasal tissues would be a promising source of diagnostic biomarkers for Alzheimer's disease. However, the amounts of amyloid β and tau are extremely small, making it difficult to quantify the levels using conventional methods such as ELISA, and thus it is challenging to utilize them for the diagnostic biomarkers.
View Article and Find Full Text PDFBiomarkers.
Alzheimers Dement
December 2024
UNAM, School of Medicine, Department of Physiology, CDMX, DF, Mexico.
Background: Mild cognitive impairment may increase the risk of Alzheimer's disease (AD) or probably accelerate the progression. AD is the most common cause of dementia, substantial neuronal loss, and neuropathological lesions can damage many brain regions. Symptoms of the disease begin with mild memory difficulties and evolve towards cognitive impairment.
View Article and Find Full Text PDFDeveloping Topics.
Alzheimers Dement
December 2024
Convergence Research Advanced Centre for Olfaction, Daegu, Korea, Republic of (South).
Background: Alzheimer's disease (AD) therapy requires timely and accurate diagnosis for prompt drug intervention, emphasizing the need for a more accessible biomarker screening test. Previous studies identified higher expression of oligomeric Aβ in AD patients, however, the quantitative measurements of nasal Aβ42 levels of the full AD continuum remain unknown.
Method: We collected nasal discharge samples from 161 subjects who underwent neuropsychological battery tests and amyloid-PET and nasal Aβ42 levels were measured via enzyme-linked immunosorbent assay (ELISA).
The different response of PM stimulated nasal epithelial spheroids in control, asthma and COPD groups.
Respir Res
January 2025
Department of Internal Medicine, Pulmonary Diseases and Allergy, Medical University of Warsaw, Banacha 1a, Warsaw, 02-097, Poland.
Background: Pathobiology of asthma and chronic obstructive pulmonary disease (COPD) is associated with changes among respiratory epithelium structure and function. Increased levels of PM from urban particulate matter (UPM) are correlated with enlarged rate of asthma and COPD morbidity as well as acute disease exacerbation. It has been suggested that pre-existing pulmonary obstructive diseases predispose epithelium for different biological response than in healthy airways.
View Article and Find Full Text PDFTissue Eosinophils Threshold and its Association with Adult-Onset Asthma in Chronic Rhinosinusitis.
Int Forum Allergy Rhinol
January 2025
Center of Excellence in Otolaryngology-Head & Neck Surgery, Rajavithi Hospital, Bangkok, Thailand.
Introduction: Tissue eosinophil counts (TEC) might serve as a biomarker linking chronic rhinosinusitis (CRS) and the presence of adult-onset asthma. This study aimed to determine if TEC in sinus mucosa/polyps in CRS patients is an independent indicator of asthma and to identify its optimal cut-off point.
Methods: This cross-sectional study was conducted on primary CRS patients scheduled for surgery.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!