[Effect of proto-oncogene protein c-met ribozyme transfection on biological behavior of gastric carcinoma cells].

Objective: To explore the effect of c-met ribozyme transfection on biological behavior of gastric carcinoma cells.

Methods: U1/met292 plasmid containing c-met ribozyme gene was transfected into L2 subline of SGC-7901 gastric cell line, and the proliferative ability, distribution of cell cycle, protein expression of VEGF and c-met, as well as the potential of liver metastasis of the transfected subline were determined.

Results: There were no significant difference in proliferative ability, distribution of cell cycle between the transfected cells and the control cells. The protein expression of VEGF and c-met, as well as the liver metastatic potential significantly decreased in the transfected cells than those in the control cells (P< 0.05).

Conclusion: The liver metastatic potential of c-met positive gastric cancer cells may be prevented by inhibiting c-met expression.