We explore the means by which immobilization of a substrate on a surface can increase the rate of a diffusion-controlled enzymatic reaction. A quasichemical approach is developed and compared with Brownian dynamics simulations. We use these methods to show that restricting only the orientation of the enzyme by long-range interactions with the surface is sufficient for enhancing catalysis.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1367114 | PMC |
| http://dx.doi.org/10.1529/biophysj.105.072785 | DOI Listing |
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