AI Article Synopsis
- Two genes, BnNHL18A and BnNHL18B, related to Arabidopsis NHL genes, were isolated from oilseed rape seedlings exposed to salt treatment.
- BnNHL18A transcript levels increase in response to various stressors, indicating its potential role in plant stress responses.
- Localization studies show BnNHL18A is primarily found in the endoplasmic reticulum, and its protein localization changes with different treatments, suggesting a shared mechanism for handling both abiotic and biotic stresses.
Article Abstract
The two genes, named BnNHL18A and BnNHL18B, showing sequence homology with Arabidopsis NDR1/HIN1-like (NHL) genes, were isolated from cDNA library prepared with oilseed rape (Brassica napus) seedlings treated with NaCl. The transcript level of BnNHL18A was increased by sodium chloride, ethephon, hydrogen peroxide, methyl jasmonate, or salicylic acid treatment. The coding regions of BnNHL18A and BnNHL18B contain a sarcolipin (SLN)-like sequence. Analysis of the localization of smGFP fusion proteins showed that BnNHL18A is mainly localized to endoplasmic reticulum (ER). This result suggests that the SLN-like sequence plays a role in retaining proteins in ER membrane in plants. In response to NaCl, hydrogen peroxide, ethephon, and salicylic acid treatments, the protein localization of BnNHL18A was changed. Our findings suggest a common function of BnNHL18A in biotic and abiotic stresses, and demonstrate the presence of the shared mechanism of protein translocalization between the responses to plant pathogen and to osmotic stress.
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| http://dx.doi.org/10.1016/j.bbrc.2005.10.210 | DOI Listing |
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BnNHL18A shows a localization change by stress-inducing chemical treatments.
Biochem Biophys Res Commun
January 2006
School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
Article Synopsis
- Two genes, BnNHL18A and BnNHL18B, related to Arabidopsis NHL genes, were isolated from oilseed rape seedlings exposed to salt treatment.
- BnNHL18A transcript levels increase in response to various stressors, indicating its potential role in plant stress responses.
- Localization studies show BnNHL18A is primarily found in the endoplasmic reticulum, and its protein localization changes with different treatments, suggesting a shared mechanism for handling both abiotic and biotic stresses.
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