A rapid and reliable diagnostic assay for acute hepatitis E virus (HEV) infection is needed. We evaluated a rapid, immunochromatographic assay for IgM antibodies to HEV (ASSURE HEV IgM Rapid Test) using acute-phase HEV samples (n = 200) from Indonesia and Nepal and convalescent-phase HEV samples (n = 70) from Nepal. Blood donors in Thailand (n = 100), individuals with hepatitis A (n = 80), hepatitis B (n = 45), and hepatitis C (n = 50) in Thailand and Nepal, acute-phase sera of individuals with Epstein-Barr virus infection (n = 20), and rheumatoid factor-positive blood (n = 26) served as negative controls. The assay had a sensitivity of 93% (95% confidence interval [CI] = 88.5-96.1%) and a specificity of 99.7% (95% CI = 98.3-100%). The positive and negative predictive values were 99.5% (95% CI = 97.1-100%) and 95.8% (95% CI = 93.1-97.7%), respectively. These results suggest that this assay is a sensitive and specific tool for the rapid diagnosis of acute HEV infection.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Electropositive Magnetic Fluorescent Nanoprobe-Mediated Immunochromatographic Assay for the Ultrasensitive and Simultaneous Detection of Bacteria.
Adv Sci (Weinh)
January 2025
Department of Clinical Laboratory Medicine, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong, 510000, China.
Immunochromatographic assays (ICAs) provide simple and rapid strategies for bacterial diagnosis but still suffer from the problems of low sensitivity and high dependency on paired antibodies. Herein, the broad-spectrum capture and detection capability of the antibody-free electropositive nanoprobe are clarified for bacteria for the first time and an ultrasensitive fluorescent ICA platform is constructed for the simultaneous diagnosis of multiple pathogens. A magnetic multilayer quantum dot nanocomposite with an amino-embedded SiO shell (MagMQD@Si) is designed to enrich bacteria from solutions effectively, offer high luminescence, and reduce background signals on test strips, thus greatly improving the sensitivity and stability of ICA technique for pathogen.
View Article and Find Full Text PDFA rapid and highly sensitive fluorescence immunochromatographic test strip for pepsin detection in human hypopharyngeal saliva.
Anal Methods
January 2025
Otolaryngology, Head and Neck Surgery Department of Fujian Provincial Hospital, Provincial Clinical Medical College of Fujian Medical University, Fuzhou University Affiliated Provincial Hospital, Fuzhou, Fujian, 35001, China.
Pepsin serves as a potential biomarker for laryngopharyngeal reflux disease (LPRD). The reported methods for detecting pepsin still have limitations, such as long reaction times and low sensitivity. Therefore, it is crucial to develop a rapid, simple and sensitive detection method.
View Article and Find Full Text PDFHapten prediction, monoclonal antibody preparation, and development of an immunochromatographic assay for the detection of fenamiphos.
J Hazard Mater
January 2025
International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, PR China. Electronic address:
Fenamiphos (FENA) is an organophosphorus insecticide, and its residues in fruits, vegetables, and the environment have raised concerns. Therefore, it is very important to develop a simple, rapid, and accurate method for FENA detection. In this study, a novel FENA hapten was designed and predicted based on computer-aided simulation technology, and high-performance anti-FENA monoclonal antibodies were screened using a matrix effect-enhanced screening method, with a half-maximal inhibitory concentration of 1.
View Article and Find Full Text PDFFluorescence immunochromatographic assay for deoxynivalenol using immunomagnetic bead purification.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess
January 2025
College of Animal Science and Technology, Beijing University of Agriculture, Beijing, China.
Deoxynivalenol (DON) contaminates various complex matrices, necessitating straightforward, effective cleanup and precise detection methods. This study employed immunomagnetic beads for sample purification and utilized a competitive time-resolved fluoro-immuno-chromatographic assay to achieve quantitative detection of DON in corn and its by-products. The limits of detection and quantification were 104 μg/kg and 243 μg/kg, respectively.
View Article and Find Full Text PDFDevelopment of colloidal gold immunochromatographic strip for rapid detection of cyvirus cyprinidallo 2.
Dis Aquat Organ
January 2025
National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, 201306 Shanghai, PR China.
Cyvirus cyprinidallo 2 (CyHV-2) is the pathogen of herpesviral hematopoietic necrosis (HVHN) that mainly infects goldfish Carassius auratus and crucian carp C. carassius and is characterized by high infectivity and pathogenicity. The availability of rapid and convenient detection methods is essential for early detection of CyHV-2.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!