The entomopathogenic fungus Metarhizium anisopliae var. acridum is registered as a mycopesticide for acridid control in Africa and Australia. Traditionally, identification of M. anisopliae var. acridum infection in grasshoppers and locusts has relied upon development of fungal growth in infected cadavers. Conventional methods of detection of this entomopathogen in the environment and non-target organisms have been based on culture and bioassay. A PCR-based method for the detection of M. anisopliae var. acridum was developed. Sequence data from the distinct ITS rDNA regions facilitated the design of PCR primers that were used in PCR-based diagnostic assays for the detection of fungal DNA. The amplified sequence was 420 bp in length and specific to M. anisopliae var. acridum. Isolates of M. anisopliae var. anisopliae and M. flavoviride produced no PCR product with these primers. Other fungal entomopathogens, plant pathogens, mycopathogens, and soil saprophytes were also not detected by the pathogen-specific primers. The assay was also effective for the detection of M. anisopliae var. acridum DNA in the presence of soil DNA extracts and in infected grasshoppers.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1017/s0953756205003746 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Distinct biological control agents differentially modulate the immune system of the sugarcane borer larvae (Diatraea saccharalis).
J Invertebr Pathol
December 2024
Departamento de Agronomia- Entomologia, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros, 52171-900 Recife, PE, Brazil; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Parque Estação Biológica - PqEB, 70770-901, Brasília, DF, Brazil. Electronic address:
The humoral response plays a crucial role in insect defense against parasites and pathogens, typically producing antimicrobial peptides through the Toll, IMD, and Jak-STAT signaling pathways, as well as melanization via phenoloxidases. However, many studies use nonpathogenic or opportunistic organisms and often infect insects in nonnatural ways, such as piercing or injecting the pathogen into the hemocoel. The objective of this study was to examine the modulation of the main humoral pathway genes involved in the interaction between the nonmodel organism Diatraea saccharalis (the sugarcane borer) and different biological control agents.
View Article and Find Full Text PDFInsecticidal potential of , and individually and their synergistic effect with barazide against .
Heliyon
September 2024
Plant Production Department, College of Food and Agricultural Sciences, King Saud University, P.O. Box. 2460, Riyadh 11451, Saudi Arabia.
Excessive use of insecticides are responsible to contaminate the environment, soil health, developing resistance in the insect pests, introduces new species, toxic to human and eliminates non-target organisms and affects the eco-balance and biodiversity adversely. Application of microbial bio-agents with the chemical insecticides is an assertive way to manage the population of pests, in an addition to dropping down the chemical residues risk to the eco-system. Larval stages of are prolific eater, caused huge losses globally.
View Article and Find Full Text PDFEvaluation of acaricidal activity in entomopathogenic fungi for poultry red mite (Dermanyssus gallinae) control.
Vet Parasitol
October 2024
Parasitic and Honeybee Disease Laboratory, Bacterial Disease Division, Department of Animal and Plant Health Research, Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea. Electronic address:
Article Synopsis
- The poultry red mite (PRM) is a significant threat to egg-laying hens, causing health issues and economic losses in the poultry industry due to their blood-feeding behavior.
- Concerns over chemical treatments for mites include resistance to miticides and potential egg contamination, leading to the exploration of biocontrol methods using entomopathogenic fungi.
- A study tested 17 fungal strains from South Korea, finding that 16 showed acaricidal properties, with Metarhizium anisopliae causing 100% mortality in mites after just five days, highlighting the potential for these fungi in effective PRM management.
Entomopathogens and Parasitoids Allied in Biocontrol: A Systematic Review.
Pathogens
July 2023
School of Agricultural, Forest and Food Sciences (HAFL), Bern University of Applied Sciences (BFH), Länggasse 85, 3052 Zollikofen, Switzerland.
Biological pest control is an environmentally friendly alternative to synthetic pesticides, using organisms such as viruses, bacteria, fungi, and parasitoids. However, efficacy is variable and combining different biocontrol agents could improve success rates. We conducted a systematic review of studies combining a parasitoid with an entomopathogenic microorganism, the first of its kind.
View Article and Find Full Text PDFScreening of Fungal Strains and Formulations of to Control in Chinese Flowering Cabbage.
Insects
June 2023
National Key Laboratory of Green Pesticide, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China.
(1) Background: The cabbage flea beetle (CFB; ) seriously damages the production of Chinese flowering cabbage (CFC; L. ssp. var.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!