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Metagenomic next-generation sequencing for the clinical identification of spinal infection-associated pathogens.
Front Cell Infect Microbiol
January 2025
Department of Spine Surgery, Fuzhou Second General Hospital, Fuzhou, Fujian, China.
Background: This study aimed to evaluate the efficacy of metagenomic next-generation sequencing (mNGS) technology for identifying pathogens associated with spinal infection (SI).
Methods: A retrospective analysis was conducted on clinical data from 193 patients with suspected SI between August 2020 and September 2024. Based on histopathological results, the patients were divided into the SI group (n=162) and the non-SI group (n=31).
Rapid detection assays for Bacillus anthracis, Yersinia pestis, and Brucella spp. via triplex-recombinase polymerase amplification.
Mol Biol Rep
January 2025
State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Biotechnology, 20 Dongdajie Street, Fengtai District, Beijing, 100071, China.
Background: Bacillus anthracis (B. anthracis), Yersinia pestis (Y. pestis), and Brucella spp.
View Article and Find Full Text PDFCamel Brucellosis: Seroprevalence, Associated Risk Factor, and Public Health Perceptions in Arero District of Borena Zone, Southern Ethiopia.
Vet Med Int
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Faculty of Veterinary Medicine, Hawassa University, P.O. Box 05, Hawassa, Ethiopia.
A cross-sectional study was conducted to determine the seroprevalence and potential risk factors of camel brucellosis and to assess public health awareness of the disease in the selected kebele of Arero District, Borena Zone, Southern Ethiopia. A total of 313 blood samples were collected from selected camels using a systematic random sampling technique. The serum samples underwent initial screening for brucellosis using the rose Bengal plate test (RBPT), with further confirmation through the indirect enzyme-linked immunosorbent Assay (i-ELISA).
View Article and Find Full Text PDFGut microbial communities and transcriptional profiles of black soldier fly (Hermitia illucens) larvae fed on fermented sericulture waste.
Waste Manag
January 2025
Guangdong Provincial Key Lab of Agro-animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China; Guangxi Key Laboratory of Sericulture Ecology and Applied Intelligent Technology/ Guangxi Collaborative Innovation Center of Modern Sericulture and Silk, Hechi University, Hechi 546300, China. Electronic address:
Sericulture waste poses significant challenges to industrial and environmental safety. Black soldier fly larvae (BSFL) offer a promising solution for organic waste management by converting it into insect protein. This study aimed to develop a microbial fermented method for utilizing sericulture waste to feed BSFL and explore the underlying mechanisms.
View Article and Find Full Text PDFTrue prevalence and spatial distribution of antibodies to Brucella spp. in goat populations in Hubei Province, People's Republic of China: Progress toward disease elimination.
Prev Vet Med
February 2025
National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China; Hubei International Scientific and Technological Cooperation Base of Veterinary Epidemiology, Huazhong Agricultural University, Wuhan, China; School of Veterinary Medicine, Murdoch University, Perth, WA, Australia. Electronic address:
Caprine brucellosis, mainly caused by Brucella melitensis, remains a significant zoonotic threat worldwide, affecting animal productivity, welfare, and public health. This study aimed to estimate the true prevalence (TP) and spatial distribution of antibodies to Brucella spp. among goat populations in Hubei Province, China.
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