Aim: To study the function of alpha-fetoprotein (AFP) in SMMC-7721 hepatoma cells.
Methods: A hairpin siRNA expressing plasmid pSilencer3.0-H1-afp was constructed and transfected into SMMC-7721 cells with Lipofectamine 2000. The expression of AFP was monitored by real-time RT-PCR and immunoassays, its effect on SMMC-7721 cell proliferation and cell death was detected by MTT and fluorescence activated cell sorter (FACS).
Results: The AFP-siRNA expressing plasmid downregulated the expression of AFP obviously (about 34%), and inhibited SMMC-7721 cell proliferation, but did not induce apoptosis.
Conclusion: Downregulation of AFP siRNA inhibits proliferation of SMMC-7721 cells, but cannot cause apoptosis.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4436734 | PMC |
| http://dx.doi.org/10.3748/wjg.v11.i38.6053 | DOI Listing |
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