[Effects of Angelica sinensis polysaccharide on cell-mediated immunity].

Aim: To investigate the effects of Angelica sinensis polysaccharide (AP) on cell-mediated immunity.

Methods: The lymphocyte proliferation was tested by MTT colormetry; T lymphocyte fraction were separated with immune magnetic beads and the change of CD4(+) T lymphocyte percentage was detected by flow cytometry.

Results: In the range of 30-300 mg/L, the total AP and its three fractions, ie AP-0, AP-1, AP-2, AP-3, directly stimulated the proliferation of murine splenocytes, and AP-3 significantly enhanced the proliferation of lymphocytes in MLR and T lymphocytes. The percentage of CD4(+) T lymphocytes in total splenocytes was also enhanced by AP-3.

Conclusion: AP can promote the cell-mediated immunity with T lymphocytes as one of its target cells.