AI Article Synopsis
- A comparison was made between an in-house Sandwich-ELISA and a commercial kit for measuring heat shock protein (Hsp) 70 in serum samples from 64 participants, showing a strong correlation in results (r = 0.807, p < 0.0001).
- The in-house method detected Hsp70 in 95% of samples, while the commercial kit only detected it in 66%, with both methods agreeing on undetectable levels in certain samples.
- The study concluded that the in-house ELISA is more cost-effective and performs well across a range of Hsp70 concentrations, despite differences in detected serum levels between the two systems.
Article Abstract
We have compared a previously developed in-house Sandwich-ELISA with a commercial kit for the determination of heat shock protein (Hsp) 70 in serum. Samples from 64 participants were tested and there was a significant correlation between results obtained using the two assays (r = 0.807, p < 0.0001). Additionally, when ranking samples on a categorical scale, the agreement was good (72%). In the commercial test system Hsp70 was detectable in 42 (66%) of the sera, compared with 61 (95%) in the in-house ELISA method. The three samples with undetectable levels of Hsp70 in the in-house ELISA were among the 22 samples with undetectable levels of Hsp70 in the commercial ELISA kit. The apparent serum concentrations detected were different in the two systems. This dissimilarity can be ascribed to differences in the matrix used. We conclude that the in-house ELISA is more economical and performs well when measuring physiologically high, as well as low, concentrations of Hsp70.
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| http://dx.doi.org/10.1016/j.jim.2005.08.012 | DOI Listing |
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