Objective: To establish HPLC chromatographic fingerprint of Citrus reticulata Blanco cv. Dahongan.
Methods: The experimental condition of the RP-HPLC method was as follows: Angilent Zorbax C18 column (4.6 mm x 250 mm, 5 microm), with linear gradient elution using (A) methanol and (B) water; The flow rate was 1.0 ml/min, and the injected volume was 20 microl, detected wavelength was 283 nm.
Results: Under the qualitative conditions we determined different samples of Citrus reticulata Blanco cv. Dahongan. There were 11 common peaks in chromatographic.
Conclusions: According to analyze and contrast the relative retention values and relative peak area in chromatographic fingerprint, we establish HPLC chromatographic fingerprint of Citrus reticulata Blanco cv. Dahongan to provide a scientific basement for the quality evaluation of it.
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