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Extraction of American ginseng polysaccharide by ultrasound-assisted deep eutectic solvents-based three-phase partitioning: Process optimization, structural characterization, and anti-ulcerative colitis study.
Ultrason Sonochem
December 2024
The Affiliated Dongguan Songshan Lake Central Hospital, Guangdong Provincial Key Laboratory of Research and Development of Natural Drugs, School of Pharmacy, Guangdong Medical University, Dongguan 523808, China. Electronic address:
Three-phase partitioning (TPP) is promising for isolating bioactive polysaccharides, but t-butanol's environmental impact limits its application. Deep eutectic solvents can serve as a green and recyclable alternative to t-butanol. This study introduces an ultrasonic-assisted DES three-phase partitioning (UA-TPP-DES) system to extract and purify American ginseng polysaccharides (AGPs).
View Article and Find Full Text PDFSimultaneous separation of the enantiomers of six anticoagulant rodenticides using chiral supercritical fluid chromatography-mass spectrometry.
J Chromatogr A
December 2024
I. U. CINQUIMA, Analytical Chemistry Group (TESEA), Dept. Analytical Chemistry, Faculty of Sciences, University of Valladolid 47011, Valladolid, Spain. Electronic address:
The simultaneous separation of the enantiomers of six anticoagulant rodenticides, derived from 4-hydroxycoumarin, has been studied in this work. Ten different stationary phases (zwitterionic, Pirkle-type, polysaccharides and macrocyclic antibiotics derivatives) were evaluated by using supercritical fluid chromatography coupled to two different detectors (circular dichroism and mass spectrometry-single quadrupole). The effect of the type of organic modifier and temperature on the chiral separation was investigated, and the best results were obtained with the column Regis S,S-Whelk-O1 at 25 °C when using a gradient elution program with methanol as organic modifier.
View Article and Find Full Text PDFComparison of novel native probiotics and paraprobiotics in modulating oxidative stress and inflammation in DSS-induced colitis: implications for enhanced therapeutic strategies in high fat diet.
BMC Immunol
December 2024
Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran.
Aim: IBD is a condition that may result from the presence of oxidative stress. The objective of this research is to evaluate and compare the potency of probiotics and paraprobiotics to modulate oxidative stress and inflammation.
Methods And Results: In the initial phase, the antioxidant capabilities of 88 strains from Lactobacillus and Bifidobacterium were evaluated.
In vitro and in vivo studies of selenium nanoparticles coated bacterial polysaccharide as anti-lung cancer agents.
Microb Cell Fact
December 2024
Department of Botany and Microbiology, Faculty of Science, Alexandria University, Alexandria, Egypt.
Microbial exopolysaccharides (EPSs) possess valuable biological functions and fascinating physicochemical properties. On the other hand, lung cancer is the primary contributor to global cancer-related deaths. However, health and safety concerns have prevented the identification and approval of any medications, including chemotherapeutic agents, for lung cancer treatment to date.
View Article and Find Full Text PDF[Bushen Huoxue Decoction regulates ADSCs-Exos to affect nucleus pulposus cell apoptosis and ERK signaling pathway in intervertebral disc degeneration].
Zhongguo Zhong Yao Za Zhi
November 2024
the First Affiliated Hospital of Hunan University of Chinese Medicine Changsha 410007, China Xiangtan Hospital of Chinese Medicine Xiangtan 411199, China.
This study aims to investigate the effects of Bushen Huoxue Decoction regulating adipose-derived stem cells(ADSCs)-exosomes(Exos) on the apoptosis of intervertebral disc nucleus pulposus cells(NPCs) and extracellular signal-regulated kinase(ERK) signaling pathway. Tert-butyl hydrogen peroxide(TBHP)-induced NPCs were divided into control, model, drug-containing serum, blank Exos, normal serum Exos, and drug-containing serum Exos groups. Cell viability and proliferation were examined by the CCK-8 assay and EdU staining, respectively.
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