AI Article Synopsis
- The nuclear cap-binding complex (CBC) plays a vital role in various processes related to mRNA metabolism by binding to the 5' cap of mRNA.
- The CBP20 subunit of CBC interacts with the mRNA through numerous hydrogen bonds and the stacking of tyrosine sidechains with the first two bases of the capped mRNA.
- Researchers created an mRNA tetramer using a new capping method, allowing them to study its affinity for CBC through fluorescence titration.
Article Abstract
Binding of mRNA 5' cap by the nuclear cap-binding complex (CBC) is crucial for a wide variety of mRNA metabolic events. The interaction involving the CBP20 subunit of CBC is mediated by numerous hydrogen bonds and by stacking of the tyrosine sidechains with two first bases of the capped mRNA. To examine a possible role of a longer mRNA chain in the CBC-cap recognition, we have synthesized an mRNA tetramer using a novel way of capping an RNA trimer and determined its affinity for CBC by fluorescence titration.
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| http://dx.doi.org/10.1081/ncn-200061898 | DOI Listing |
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